来自红螺菌的一氧化碳脱氢酶。
Carbon monoxide dehydrogenase from Rhodospirillum rubrum.
作者信息
Bonam D, Murrell S A, Ludden P W
出版信息
J Bacteriol. 1984 Aug;159(2):693-9. doi: 10.1128/jb.159.2.693-699.1984.
Abstract
The carbon monoxide dehydrogenase from the photosynthetic bacterium Rhodospirillum rubrum was purified over 600-fold by DEAE-cellulose chromatography, heat treatment, hydroxylapatite chromatography, and preparative scale gel electrophoresis. In vitro, this enzyme catalyzed a two-electron oxidation of CO to form CO2 as the product. The reaction was dependent on the addition of an electron acceptor. The enzyme was oxygen labile, heat stable, and resistant to tryptic and chymotryptic digestion. Optimum in vitro activity occurred at pH 10.0. A sensitive, hemoglobin-based assay for measuring dissolved CO levels is presented. The in vitro Km for CO was determined to be 110 microM. CO, through an unknown mechanism, stimulated hydrogen evolution in whole cells, suggesting the presence of a reversible hydrogenase in R. rubrum which is CO insensitive in vivo.
摘要
通过DEAE-纤维素色谱法、热处理、羟基磷灰石色谱法和制备规模凝胶电泳，对光合细菌红螺菌的一氧化碳脱氢酶进行了600多倍的纯化。在体外，这种酶催化CO的双电子氧化反应，生成产物CO2。该反应依赖于电子受体的添加。该酶对氧气敏感，对热稳定，且对胰蛋白酶和糜蛋白酶消化具有抗性。体外最佳活性出现在pH 10.0。本文介绍了一种基于血红蛋白的灵敏测定溶解CO水平的方法。测定了CO在体外的Km值为110微摩尔。CO通过未知机制刺激全细胞中的氢气释放，这表明红螺菌中存在一种对体内CO不敏感的可逆氢化酶。
相似文献
1
Carbon monoxide dehydrogenase from Rhodospirillum rubrum.来自红螺菌的一氧化碳脱氢酶。
J Bacteriol. 1984 Aug;159(2):693-9. doi: 10.1128/jb.159.2.693-699.1984.
2
Characterization of the CO oxidation/H2 evolution system of Rhodospirillum rubrum. Role of a 22-kDa iron-sulfur protein in mediating electron transfer between carbon monoxide dehydrogenase and hydrogenase.深红红螺菌一氧化碳氧化/氢气产生系统的特性。一种22 kDa铁硫蛋白在介导一氧化碳脱氢酶和氢化酶之间电子传递中的作用。
J Biol Chem. 1991 Sep 25;266(27):18395-403.
3
4
Reactivity of carbon monoxide dehydrogenase from Rhodospirillum rubrum with carbon dioxide, carbonyl sulfide, and carbon disulfide.
Biochemistry. 1995 Apr 25;34(16):5372-8. doi: 10.1021/bi00016a008.
5
Nickel-deficient carbon monoxide dehydrogenase from Rhodospirillum rubrum: in vivo and in vitro activation by exogenous nickel.
Proc Natl Acad Sci U S A. 1988 Jan;85(1):31-5. doi: 10.1073/pnas.85.1.31.
6
Regulation of carbon monoxide dehydrogenase and hydrogenase in Rhodospirillum rubrum: effects of CO and oxygen on synthesis and activity.红螺菌中一氧化碳脱氢酶和氢化酶的调控：一氧化碳和氧气对合成及活性的影响。
J Bacteriol. 1989 Jun;171(6):3102-7. doi: 10.1128/jb.171.6.3102-3107.1989.
7
Nickel is required for the transfer of electrons from carbon monoxide to the iron-sulfur center(s) of carbon monoxide dehydrogenase from Rhodospirillum rubrum.镍是红螺菌一氧化碳脱氢酶将电子从一氧化碳转移至铁硫中心所必需的。
Biochemistry. 1989 Jun 13;28(12):4968-73. doi: 10.1021/bi00438a010.
8
Carbon monoxide dehydrogenase from Rhodospirillum rubrum produces formate.来自红螺菌的一氧化碳脱氢酶可产生甲酸。
J Biol Inorg Chem. 2002 Sep;7(7-8):810-4. doi: 10.1007/s00775-002-0365-z. Epub 2002 Apr 30.
9
New insights into the mechanism of nickel insertion into carbon monoxide dehydrogenase: analysis of Rhodospirillum rubrum carbon monoxide dehydrogenase variants with substituted ligands to the [Fe3S4] portion of the active-site C-cluster.镍插入一氧化碳脱氢酶机制的新见解：对活性位点C簇[Fe3S4]部分配体被取代的红螺菌一氧化碳脱氢酶变体的分析
J Biol Inorg Chem. 2005 Dec;10(8):903-12. doi: 10.1007/s00775-005-0043-z. Epub 2005 Nov 8.
10
CO-dependent H2 evolution by Rhodospirillum rubrum: role of CODH:CooF complex.红螺菌依赖一氧化碳的氢气进化：一氧化碳脱氢酶：CooF复合物的作用
Biochim Biophys Acta. 2006 Dec;1757(12):1582-91. doi: 10.1016/j.bbabio.2006.10.003. Epub 2006 Oct 14.
引用本文的文献
1
Current status of carbon monoxide dehydrogenases (CODH) and their potential for electrochemical applications.一氧化碳脱氢酶（CODH）的当前研究现状及其在电化学应用中的潜力。
Bioresour Bioprocess. 2023 Nov 27;10(1):84. doi: 10.1186/s40643-023-00705-9.
2
Second and Outer Coordination Sphere Effects in Nitrogenase, Hydrogenase, Formate Dehydrogenase, and CO Dehydrogenase.二配位和外配位球效应对氮酶、氢化酶、甲酸脱氢酶和一氧化碳脱氢酶的影响。
Chem Rev. 2022 Jul 27;122(14):11900-11973. doi: 10.1021/acs.chemrev.1c00914. Epub 2022 Jul 18.
3
Synthesis Gas (Syngas)-Derived Medium-Chain-Length Polyhydroxyalkanoate Synthesis in Engineered Rhodospirillum rubrum.工程化红螺菌中合成气衍生的中链长度聚羟基脂肪酸酯的合成
Appl Environ Microbiol. 2016 Sep 30;82(20):6132-6140. doi: 10.1128/AEM.01744-16. Print 2016 Oct 15.
4
CO2 Reduction Catalyzed by Nitrogenase: Pathways to Formate, Carbon Monoxide, and Methane.固氮酶催化的二氧化碳还原：生成甲酸、一氧化碳和甲烷的途径。
Inorg Chem. 2016 Sep 6;55(17):8321-30. doi: 10.1021/acs.inorgchem.6b00388. Epub 2016 Aug 8.
5
Metabolic response of Clostridium ljungdahlii to oxygen exposure.尤氏梭菌对氧气暴露的代谢反应。
Appl Environ Microbiol. 2015 Dec;81(24):8379-91. doi: 10.1128/AEM.02491-15. Epub 2015 Oct 2.
6
Genome annotation provides insight into carbon monoxide and hydrogen metabolism in Rubrivivax gelatinosus.基因组注释有助于深入了解嗜胶红杆菌中的一氧化碳和氢代谢。
PLoS One. 2014 Dec 5;9(12):e114551. doi: 10.1371/journal.pone.0114551. eCollection 2014.
7
Sustaining N2-dependent growth in the presence of CO.在有 CO 的情况下维持 N2 依赖性生长。
J Bacteriol. 2011 Feb;193(3):774-7. doi: 10.1128/JB.00794-10. Epub 2010 Nov 29.
8
A "radical dance" in thiamin biosynthesis: mechanistic analysis of the bacterial hydroxymethylpyrimidine phosphate synthase.硫胺素生物合成中的“激进舞蹈”：细菌磷酸羟甲基嘧啶合酶的机制分析
Angew Chem Int Ed Engl. 2010 Nov 8;49(46):8653-6. doi: 10.1002/anie.201003419.
9
Characterization of genes responsible for the CO-linked hydrogen production pathway in Rubrivivax gelatinosus.鉴定Rubrivivax gelatinosus 中与 CO 偶联产氢途径相关的基因。
Appl Environ Microbiol. 2010 Jun;76(11):3715-22. doi: 10.1128/AEM.02753-09. Epub 2010 Apr 16.
10
Factors Limiting Aliphatic Chlorocarbon Degradation by Nitrosomonas europaea: Cometabolic Inactivation of Ammonia Monooxygenase and Substrate Specificity.限制硝酸单胞菌降解脂肪族氯代烃的因素：氨单加氧酶的共代谢失活和底物特异性。
Appl Environ Microbiol. 1991 Oct;57(10):2986-94. doi: 10.1128/aem.57.10.2986-2994.1991.
本文引用的文献
1
Growth of Eubacterium limosum with Carbon Monoxide as the Energy Source.以一氧化碳为能源的迟缓真杆菌生长。
Appl Environ Microbiol. 1982 Jan;43(1):70-4. doi: 10.1128/aem.43.1.70-74.1982.
2
Light-dependent utilization of organic compounds and photoproduction of molecular hydrogen by photosynthetic bacteria; relationships with nitrogen metabolism.光合细菌对有机化合物的光依赖利用及分子氢的光产生；与氮代谢的关系。
Arch Biochem Biophys. 1961 Sep;94:449-63. doi: 10.1016/0003-9861(61)90073-x.
3
Enzymic oxidation of carbon monoxide.一氧化碳的酶促氧化
Biochim Biophys Acta. 1958 Oct;30(1):194-5. doi: 10.1016/0006-3002(58)90263-4.
4
Carbon monoxide:methylene blue oxidoreductase from Pseudomonas carboxydovorans.一氧化碳：来自食羧假单胞菌的亚甲蓝氧化还原酶。
J Bacteriol. 1980 Jan;141(1):74-80. doi: 10.1128/jb.141.1.74-80.1980.
5
6
Carbon monoxide metabolism of the methylotrophic acidogen Butyribacterium methylotrophicum.甲基营养产酸菌嗜甲基丁酸杆菌的一氧化碳代谢
J Bacteriol. 1982 Jan;149(1):255-63. doi: 10.1128/jb.149.1.255-263.1982.
7
Occurrence of nickel in carbon monoxide dehydrogenase from Clostridium pasteurianum and Clostridium thermoaceticum.巴氏梭菌和热醋酸梭菌一氧化碳脱氢酶中镍的存在情况。
J Bacteriol. 1982 Feb;149(2):561-6. doi: 10.1128/jb.149.2.561-566.1982.
8
Synthesis of acetyl coenzyme A from carbon monoxide, methyltetrahydrofolate, and coenzyme A by enzymes from Clostridium thermoaceticum.利用热醋梭菌的酶从一氧化碳、甲基四氢叶酸和辅酶A合成乙酰辅酶A。
J Bacteriol. 1982 Feb;149(2):440-8. doi: 10.1128/jb.149.2.440-448.1982.
9
Purification and some properties of carbon monoxide dehydrogenase from Pseudomonas carboxydohydrogena.来自羧基氢假单胞菌的一氧化碳脱氢酶的纯化及某些性质
J Bacteriol. 1981 Dec;148(3):904-11. doi: 10.1128/jb.148.3.904-911.1981.
10
Purification of carbon monoxide dehydrogenase, a nickel enzyme from Clostridium thermocaceticum.一氧化碳脱氢酶的纯化，一种来自热醋梭菌的镍酶。
J Biol Chem. 1980 Aug 10;255(15):7174-80.
Zotero 插件