Johnson D J, Ostlund E N, Pedersen D D, Schmitt B J
Animal and Plant Health Inspection Service, U.S. Department of Agriculture, 1800 Dayton Ave., Ames, IA 50010-0844, USA.
Emerg Infect Dis. 2001 Jul-Aug;7(4):739-41. doi: 10.3201/eid0704.010425.
A traditional single-stage reverse transcription-polymerase chain reaction (RT-PCR) procedure is effective in determining West Nile (WN) virus in avian tissue and infected cell cultures. However, the procedure lacks the sensitivity to detect WN virus in equine tissue. We describe an RT-nested PCR (RT-nPCR) procedure that identifies the North American strain of WN virus directly in equine and avian tissues.
传统的单阶段逆转录-聚合酶链反应(RT-PCR)程序在检测禽类组织和受感染细胞培养物中的西尼罗河(WN)病毒方面是有效的。然而,该程序缺乏在马组织中检测WN病毒的灵敏度。我们描述了一种RT巢式PCR(RT-nPCR)程序,该程序可直接在马和禽类组织中鉴定北美WN病毒株。