Ebel G D, Dupuis A P, Ngo K, Nicholas D, Kauffman E, Jones S A, Young D, Maffei J, Shi P Y, Bernard K, Kramer L D
Arbovirus Laboratories, New York State Department of Health, 5668 State Farm Road, Slingerlands, NY 12159, USA.
Emerg Infect Dis. 2001 Jul-Aug;7(4):650-3. doi: 10.3201/eid0704.010408.
We analyzed nucleotide sequences from the envelope gene of 11 West Nile (WN) virus strains collected in New York State during the 2000 transmission season to determine whether they differed genetically from each other and from the initial strain isolated in 1999. The complete envelope genes of these strains were amplified by reverse transcription-polymerase chain reaction. The resulting sequences were aligned, the genetic distances were computed, and a phylogenetic tree was constructed. Ten (0.7%) of 1,503 positions in the envelope gene were polymorphic in one or more sequences. The genetic distances were 0.003 or less. WN virus strains circulating in 2000 were homogeneous with respect to one another and to a strain isolated in 1999.
我们分析了2000年传播季节在纽约州采集的11株西尼罗河(WN)病毒毒株包膜基因的核苷酸序列,以确定它们彼此之间以及与1999年分离出的初始毒株在基因上是否存在差异。通过逆转录-聚合酶链反应扩增这些毒株的完整包膜基因。对所得序列进行比对,计算遗传距离,并构建系统发育树。包膜基因1503个位置中的10个(0.7%)在一个或多个序列中存在多态性。遗传距离为0.003或更小。2000年流行的WN病毒毒株彼此之间以及与1999年分离出的毒株具有同质性。