Tawab M A, Kaunzinger A, Bahr U, Karas M, Wurglics M, Schubert-Zsilavecz M
Institute of Pharmaceutical Chemistry, Johann Wolfgang Goethe University, Frankfurt/Main, Germany.
J Chromatogr B Biomed Sci Appl. 2001 Sep 25;761(2):221-7. doi: 10.1016/s0378-4347(01)00335-8.
A validated HPLC method for the determination of 11-keto-beta-boswellic acid (KBA) in human plasma was developed. The method involves the solid-phase extraction of KBA from plasma followed by a separation with reversed-phase HPLC. Calibration was based on external standardisation and ranged between 0.1 and 2.0 microg KBA per ml plasma. Linearity was established over the entire calibration range and in each case the coefficient of correlation (r2) was above 0.99. The recovery of KBA from plasma was 85.7%. It was further demonstrated that the method can be applied successfully to monitor the level of KBA in plasma.
建立了一种经验证的高效液相色谱法（HPLC）用于测定人血浆中11-酮-β-乳香酸（KBA）。该方法包括从血浆中固相萃取KBA，然后用反相高效液相色谱法进行分离。校准基于外标法，血浆中KBA的浓度范围为每毫升0.1至2.0微克。在整个校准范围内均建立了线性关系，每种情况下相关系数（r2）均高于0.99。KBA从血浆中的回收率为85.7%。进一步证明该方法可成功应用于监测血浆中KBA的水平。
