Raja Alsaba F, Ali Furqan, Khan Inshad A, Shawl Abdul S, Arora Daljit S
Microbiology Unit, Indian Institute of Integrative Medicine (CSIR), Sanatnagar, Srinagar, 190005, India.
BMC Res Notes. 2011 Oct 13;4:406. doi: 10.1186/1756-0500-4-406.
Boswellic acids mixture of triterpenic acids obtained from the oleo gum resin of Boswellia serrata and known for its effectiveness in the treatment of chronic inflammatory disease including peritumor edema. Boswellic acids have been extensively studied for a number of activities including anti inflammatory, antitumor, immunomodulatory, and inflammatory bowel diseases. The present study describes the antimicrobial activities of boswellic acid molecules against oral cavity pathogens. Acetyl-11-keto-β-boswellic acid (AKBA), which exhibited the most potent antibacterial activity, was further evaluated in time kill studies, mutation prevention frequency, postantibiotic effect (PAE) and biofilm susceptibility assay against oral cavity pathogens.
AKBA exhibited an inhibitory effect on all the oral cavity pathogens tested (MIC of 2-4 μg/ml). It exhibited concentration dependent killing of Streptococcus mutans ATCC 25175 up to 8 × MIC and also prevented the emergence of mutants of S.mutans ATCC 25175 at 8× MIC. AKBA demonstrated postantibiotic effect (PAE) of 5.7 ± 0.1 h at 2 × MIC. Furthermore, AKBA inhibited the formation of biofilms generated by S.mutans and Actinomyces viscosus and also reduced the preformed biofilms by these bacteria.
AKBA can be useful compound for the development of antibacterial agent against oral pathogens and it has great potential for use in mouthwash for preventing and treating oral infections.
乳香酸是从锯叶乳香的油胶树脂中获得的三萜酸混合物，以其在治疗包括肿瘤周围水肿在内的慢性炎症性疾病方面的有效性而闻名。乳香酸已针对多种活性进行了广泛研究，包括抗炎、抗肿瘤、免疫调节和炎症性肠病。本研究描述了乳香酸分子对口腔病原体的抗菌活性。对表现出最有效抗菌活性的乙酰 - 11 - 酮 - β - 乳香酸（AKBA），进一步进行了时间杀菌研究、突变预防频率、抗生素后效应（PAE）以及针对口腔病原体的生物膜敏感性测定。
AKBA 对所有测试的口腔病原体均表现出抑制作用（MIC 为 2 - 4 μg/ml）。它对变形链球菌 ATCC 25175 表现出浓度依赖性杀菌作用，最高可达 8×MIC，并且在 8×MIC 时还可防止变形链球菌 ATCC 25175 突变体的出现。AKBA 在 2×MIC 时显示出 5.7±0.1 小时的抗生素后效应（PAE）。此外，AKBA 抑制了变形链球菌和粘性放线菌形成的生物膜，并且还减少了这些细菌预先形成的生物膜。
AKBA 可能是开发抗口腔病原体抗菌剂的有用化合物，并且在用于预防和治疗口腔感染的漱口水中具有巨大的应用潜力。
