Galán Martín A M, Mariño J I, García de Tiedra M P, Marabé J J, Caballero Loscos M J, Mariño M M
Department of Analytical Chemistry and Electrochemistry, Faculty of Sciences, Extremadura University, Badajoz, Spain.
J Chromatogr B Biomed Sci Appl. 2001 Sep 25;761(2):229-36. doi: 10.1016/s0378-4347(01)00338-3.
Metabolites of nandrolone were determined in the urine of several sportsmen, sedentary and post-menopausal women by capillary gas chromatography-mass spectrometry quadrupole (GC-MS) and capillary gas chromatography mass-mass spectrometry ion trap (GC-MS-MS) methods. The method employed was GC-EI-MS with 17alpha-methyltestosterone as internal standard with ethyl ether extraction prior to selected ion monitoring of the bis(trimethylsilyl) ethers at ion masses m/z 405 and 420 for the nandrolone metabolites, and 418 and 403 for nandrolone derivative. Recovery for nandrolone, 19-norandrosterone (19-NA) and 19-noretiocholanolone (19-NE) was 97.20, 94.17 and 95.54%, respectively. Detection limits for nandrolone, 19-NA and 19-NE were 0.03, 0.01 and 0.06 ng/ml. Metabolites of nandrolone (19-NA and 19-NE) were found in 12.5% (n = 40) of sportsmen and 40% (n = 10) of post-menopausal women.
采用毛细管气相色谱 - 四极杆质谱联用（GC - MS）和毛细管气相色谱 - 离子阱质谱 - 质谱联用（GC - MS - MS）方法，测定了数名运动员、久坐不动的人和绝经后女性尿液中的诺龙代谢物。所采用的方法是GC - EI - MS，以17α - 甲基睾酮为内标，在对诺龙代谢物的双（三甲基硅基）醚进行选择离子监测时，离子质量为m/z 405和420，对诺龙衍生物则为418和403，采用乙醚萃取。诺龙、19 - 去甲雄酮（19 - NA）和19 - 去甲本胆烷醇酮（19 - NE）的回收率分别为97.20%、94.17%和95.54%。诺龙、19 - NA和19 - NE的检测限分别为0.03、0.01和0.06 ng/ml。在12.5%（n = 40）的运动员和40%（n = 10）的绝经后女性中发现了诺龙代谢物（19 - NA和19 - NE）。
