Nuttall T J, Lamb J R, Hill P B
University of Edinburgh Hospital for Small Animals, Easter Bush Veterinary Centre, Easter Bush, Midlothian.
Res Vet Sci. 2001 Aug;71(1):51-7. doi: 10.1053/rvsc.2001.0485.
Atopic dermatitis is a well-recognised chronic inflammatory skin disease of humans and dogs. Most atopic dogs are sensitised to Dermatophagoides mites. The aim of this study was to characterise allergens in different Dermatophagoides species using polyclonal and monoclonal antibodies to canine IgE. Western blots were prepared from crude extracts of D farinae, D pteronyssinus and D microceras, and purified group 1 and 2 allergens under reducing and non-reducing conditions. They were probed with sera from atopic (n = 33) and healthy (n = 27) dogs. There was no significant difference in the sensitivity or specificity between the polyclonal and monoclonal sera in detecting Dermatophagoides -specific IgE. Major allergens common to both D farinae and D pteronyssinus were detected at 97-98 kDa, 103-104 kDa and 134-139 kDa on both reducing and non-reducing blots. Major allergens at 84-85 kDa, 65-69 kDa and 44-45 kDa were only recognised on reducing blots, suggesting that these are fragments of the larger allergens. Only a few sera recognised group 1 or 2 allergens on blots of crude extracts or purified allergens. These results confirm that, in atopic dogs, high molecular weight allergens are the most important Dermatophagoides allergens, rather than the low molecular weight group 1 and 2 proteins.
特应性皮炎是一种在人类和犬类中广为人知的慢性炎症性皮肤病。大多数患特应性皮炎的犬对尘螨过敏。本研究的目的是使用针对犬IgE的多克隆和单克隆抗体来鉴定不同尘螨物种中的过敏原。用粉尘螨、屋尘螨和梅氏嗜霉螨的粗提物以及纯化的1组和2组过敏原在还原和非还原条件下制备蛋白质免疫印迹。用患特应性皮炎的犬（n = 33）和健康犬（n = 27）的血清对其进行检测。在检测尘螨特异性IgE方面，多克隆和单克隆血清之间的敏感性或特异性没有显著差异。在还原和非还原印迹上均检测到粉尘螨和屋尘螨共有的主要过敏原，分子量分别为97 - 98 kDa、103 - 104 kDa和134 - 139 kDa。分子量为84 - 85 kDa、65 - 69 kDa和44 - 45 kDa的主要过敏原仅在还原印迹上被识别，这表明它们是较大过敏原的片段。只有少数血清在粗提物或纯化过敏原的印迹上识别出1组或2组过敏原。这些结果证实，在患特应性皮炎的犬中，高分子量过敏原是最重要的尘螨过敏原，而非低分子量的1组和2组蛋白质。
