Fleming N, Brown D, Balls M
J Cell Sci. 1975 Aug;18(3):533-44. doi: 10.1242/jcs.18.3.533.
Fragments of liver from the adult urodele Amphiuma means, the Congo eel, were maintained in organ culture for up to 70 days. The normal electrophoretic patterns of several enzymes were retained. The activities of ornithine transcarbamylase, arginase, glutamate oxalacetate transaminase and glutamate pyruvate transaminase, and urea production, glucose uptake and tissue glycogen content remained relatively constant throughout the culture period. Histological organization and hepatocyte ultrastructure were also retained. Liver fragments survived better in media based on MEM or BME than in medium based on Leibovitz L15. Since many aspects of tissue-specific structure and function are retained, long-term amphibian organ culture is well suited to studies on the control of hepatocyte function and on the effects of metabolites, hormones, drugs and toxins.
成年有尾两栖动物美洲鳗螈（Amphiuma means，即刚果鳗）的肝脏碎片在器官培养中维持了长达70天。几种酶的正常电泳图谱得以保留。在整个培养期间，鸟氨酸转氨甲酰酶、精氨酸酶、谷氨酸草酰乙酸转氨酶和谷氨酸丙酮酸转氨酶的活性以及尿素生成、葡萄糖摄取和组织糖原含量保持相对恒定。组织学结构和肝细胞超微结构也得以保留。肝脏碎片在基于MEM或BME的培养基中比在基于Leibovitz L15的培养基中存活得更好。由于组织特异性结构和功能的许多方面得以保留，长期两栖动物器官培养非常适合用于研究肝细胞功能的调控以及代谢物、激素、药物和毒素的作用。
