细胞因子对从骨巨细胞瘤分离的细胞体外骨吸收的影响。

Effect of cytokines on in vitro bone resorption by cells isolated from giant cell tumor of bone.

作者信息

Wen J, Xie D, Yao J, Zhang M, Bi J

机构信息

Department of Pathology, Sun Yat-Sen University of Medical Sciences, Guangzhou 510089, China.

出版信息

Chin Med J (Engl). 1999 May;112(5):443-7.

PMID:11593516

Abstract

OBJECTIVE

To investigate the effect of cytokines on in vitro bone resorption by cells isolated from giant cell tumor of bone.

METHODS

Mononuclear stromal cells and multinucleated giant cells (MGC) were isolated from 11 cases of giant cell tumor of bone (GCT) and their bone resorption capability in an in vitro cell-bone resorption model were tested. Expressions of some cytokines were detected by immunohistochemistry, Western blotting analysis and Enzyme-linked immunoabsorbent assay (ELISA) in the GCT.

RESULTS

The results showed that MGCs of GCT had capability to resorb bone matrix directly. Fibroblast-like stromal cells (FC) could not only resorb bone matrix directly, but also secret unknown factors to facilitate bone resorption of MGC. Exogenous TNF-alpha could significantly increase the bone resorption by both kinds of stromal cells, while exogenous IL-1 did not. Expression rate of M-CSF and level of TNF-alpha in GCT were higher than in osteosarcoma and normal serum.

CONCLUSIONS

The characteristic bone resorption behavior of GCT might be caused by its three major cell components. The M-CSF and TNF-alpha could promote their bone resorption capability.

摘要

目的

研究细胞因子对从骨巨细胞瘤分离的细胞体外骨吸收的影响。

方法

从11例骨巨细胞瘤（GCT）中分离出单核基质细胞和多核巨细胞（MGC），并在体外细胞-骨吸收模型中测试它们的骨吸收能力。通过免疫组织化学、蛋白质印迹分析和酶联免疫吸附测定（ELISA）检测GCT中一些细胞因子的表达。

结果

结果表明，GCT的MGC具有直接吸收骨基质的能力。成纤维细胞样基质细胞（FC）不仅可以直接吸收骨基质，还能分泌未知因子促进MGC的骨吸收。外源性肿瘤坏死因子-α（TNF-α）可显著增加两种基质细胞的骨吸收，而外源性白细胞介素-1（IL-1）则无此作用。GCT中巨噬细胞集落刺激因子（M-CSF）的表达率和TNF-α水平高于骨肉瘤和正常血清。