Kudo Naoko, Ogose Akira, Ariizumi Takashi, Kawashima Hiroyuki, Hotta Tetsuo, Hatano Hiroshi, Morita Tetsuro, Nagata Masaki, Siki Yukie, Kawai Akira, Hotta Yuko, Hoshino Makiko, Endo Naoto
Division of Orthopedic Surgery, Department of Regenerative Transplant Medicine, Niigata University Graduate School of Medical and Dental Sciences, Niigata City, Niigata 951-8510, Japan.
Anticancer Res. 2009 Jun;29(6):2219-25.
A giant cell tumor (GCT) of bone is a locally aggressive tumor with a propensity for local recurrence. A characteristic pattern of peripheral bone formation has been described in GCT recurrence in soft tissue, and in some pulmonary metastases from benign GCT. Although the bone formation in GCT in supposedly due to bone morphogenetic proteins (BMPs), the expression pattern of BMPs in GCT has not been well investigated.
The expression of BMPs in GCT tissues, cultured stromal cells from GCT, and osteoclast-like giant cells harvested by laser microdissection (LM), as well as from control osteosarcoma (NOS-1) cells was analyzed using reverse transcriptional-semiquantitative PCR.
BMP 2, 3, 4, 5 and 6 were expressed in the GCT tissue. The cultured GCT cells expressed BMP 2, 4, 5 and 6. The osteoclast-like giant cells expressed BMP 2, 3, 5 and 6 and BMP 5 was expressed at the highest level.
Both stromal cells and osteoclast-like cells in GCT expressed several kinds of BMPs.
骨巨细胞瘤（GCT）是一种具有局部复发倾向的局部侵袭性肿瘤。在软组织中的GCT复发以及一些良性GCT的肺转移中，已描述了外周骨形成的特征模式。尽管GCT中的骨形成被认为是由于骨形态发生蛋白（BMP）所致，但BMP在GCT中的表达模式尚未得到充分研究。
使用逆转录-半定量PCR分析BMP在GCT组织、GCT培养的基质细胞以及通过激光显微切割（LM）收获的破骨细胞样巨细胞中的表达，以及对照骨肉瘤（NOS-1）细胞中的表达。
BMP 2、3、4、5和6在GCT组织中表达。培养的GCT细胞表达BMP 2、4、5和6。破骨细胞样巨细胞表达BMP 2、3、5和6，且BMP 5表达水平最高。
GCT中的基质细胞和破骨细胞样细胞均表达多种BMP。
