用体视学方法测量青蛙骨骼肌中各组分的大小。
Sizes of components in frog skeletal muscle measured by methods of stereology.
作者信息
Mobley B A, Eisenberg B R
出版信息
J Gen Physiol. 1975 Jul;66(1):31-45. doi: 10.1085/jgp.66.1.31.
Stereological techniques of point and intersection counting were used to measure morphological parameters from light and electron micrographs of frog skeletal muscle. Results for sartorius muscle are as follows: myofibrils comprise 83% of fiber volume; their surface to volume ratio is 3.8 mum-1. Mitochondria comprise 1.6% of fiber volume. Transverse tubules comprise 0.32% of fiber volume, and their surface area per volume of fiber is 0.22 mum-1. Terminal cisternae of the sarcoplasmic reticulum comprise 4.1% of fiber volume; their surface area per volume of fiber is 0.54 mum-1. Longitudinal sarcoplasmic reticullum comprises 5.0% of fiber volume, and its surface area per volume of fiber is 1.48 mum-1. Longitudinal bridges between terminal cisternae on either side of a Z disk were observed infrequently; they make up only 0.035% of fiber volume and their surface area per volume of fiber is 0.009 mum-1. T-SR junction occurs over 67% of the surface of transverse tubules and over 27% of the surface of terminal cisternae. The surface to volume ratio of the caveolae is 48 mum-1; caveolae may increase the sarcolemmal surface area by 47%. Essentially the same results were obtained from semitendinosus fibers.
采用点计数和交点计数的体视学技术，从青蛙骨骼肌的光学和电子显微镜照片中测量形态学参数。缝匠肌的结果如下：肌原纤维占纤维体积的83%；其表面积与体积之比为3.8μm⁻¹。线粒体占纤维体积的1.6%。横管占纤维体积的0.32%，其每纤维体积的表面积为0.22μm⁻¹。肌浆网终池占纤维体积的4.1%；其每纤维体积的表面积为0.54μm⁻¹。纵行肌浆网占纤维体积的5.0%，其每纤维体积的表面积为1.48μm⁻¹。Z盘两侧终池之间的纵桥很少见；它们仅占纤维体积的0.035%，其每纤维体积的表面积为0.009μm⁻¹。横管-肌浆网连接出现在横管表面的67%以上和终池表面的27%以上。小窝的表面积与体积之比为48μm⁻¹；小窝可使肌膜表面积增加47%。从半腱肌纤维获得的结果基本相同。
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