拟南芥中一种定位于高尔基体的GDP-甘露糖转运蛋白GONST1的鉴定与特性分析
Identification and characterization of GONST1, a golgi-localized GDP-mannose transporter in Arabidopsis.
作者信息
Baldwin T C, Handford M G, Yuseff M I, Orellana A, Dupree P
机构信息
Department of Biochemistry, University of Cambridge, Building O, Downing Site, Cambridge CB2 1QW, United Kingdom.
出版信息
Plant Cell. 2001 Oct;13(10):2283-95. doi: 10.1105/tpc.010247.
Abstract
Transport of nucleotide sugars across the Golgi apparatus membrane is required for the luminal synthesis of a variety of plant cell surface components. We identified an Arabidopsis gene encoding a nucleotide sugar transporter (designated GONST1) that we have shown by transient gene expression to be localized to the Golgi. GONST1 complemented a GDP-mannose transport-defective yeast mutant (vrg4-2), and Golgi-rich vesicles from the complemented strain displayed increased GDP-mannose transport activity. GONST1 promoter::beta-glucuronidase studies suggested that this gene is expressed ubiquitously. The identification of a Golgi-localized nucleotide sugar transporter from plants will allow the study of the importance of this class of proteins in the synthesis of plant cell surface components such as cell wall polysaccharides.
摘要
多种植物细胞表面成分的腔内合成需要核苷酸糖跨高尔基体膜运输。我们鉴定出拟南芥中一个编码核苷酸糖转运蛋白的基因（命名为GONST1），通过瞬时基因表达我们已证明它定位于高尔基体。GONST1弥补了GDP-甘露糖转运缺陷型酵母突变体（vrg4-2），并且来自互补菌株的富含高尔基体的囊泡显示出增加的GDP-甘露糖转运活性。GONST1启动子::β-葡萄糖醛酸酶研究表明该基因在植物中普遍表达。从植物中鉴定出一种定位于高尔基体的核苷酸糖转运蛋白，将有助于研究这类蛋白质在植物细胞表面成分（如细胞壁多糖）合成中的重要性。
相似文献
1
Identification and characterization of GONST1, a golgi-localized GDP-mannose transporter in Arabidopsis.拟南芥中一种定位于高尔基体的GDP-甘露糖转运蛋白GONST1的鉴定与特性分析
Plant Cell. 2001 Oct;13(10):2283-95. doi: 10.1105/tpc.010247.
2
Arabidopsis thaliana expresses multiple Golgi-localised nucleotide-sugar transporters related to GONST1.拟南芥表达多种与GONST1相关的定位于高尔基体的核苷酸糖转运蛋白。
Mol Genet Genomics. 2004 Nov;272(4):397-410. doi: 10.1007/s00438-004-1071-z. Epub 2004 Oct 8.
3
Abnormal glycosphingolipid mannosylation triggers salicylic acid-mediated responses in Arabidopsis.异常糖脂甘露糖基化触发拟南芥中水杨酸介导的反应。
Plant Cell. 2013 May;25(5):1881-94. doi: 10.1105/tpc.113.111500. Epub 2013 May 21.
4
Identification of a conserved motif in the yeast golgi GDP-mannose transporter required for binding to nucleotide sugar.鉴定酵母高尔基体GDP-甘露糖转运蛋白中与核苷酸糖结合所需的保守基序。
J Biol Chem. 2001 Feb 9;276(6):4424-32. doi: 10.1074/jbc.M009114200. Epub 2000 Nov 6.
5
Identification of a Candida glabrata homologue of the S. cerevisiae VRG4 gene, encoding the Golgi GDP-mannose transporter.酿酒酵母VRG4基因（编码高尔基体GDP-甘露糖转运蛋白）的光滑念珠菌同源物的鉴定。
Yeast. 2002 Jun 15;19(8):691-8. doi: 10.1002/yea.854.
6
Class 1 reversibly glycosylated polypeptides are plasmodesmal-associated proteins delivered to plasmodesmata via the golgi apparatus.1类可逆糖基化多肽是通过高尔基体传递到胞间连丝的胞间连丝相关蛋白。
Plant Cell. 2005 Jun;17(6):1788-800. doi: 10.1105/tpc.105.031823. Epub 2005 May 6.
7
The GDP-mannose transporter gene (DoGMT) from Dendrobium officinale is critical for mannan biosynthesis in plant growth and development.铁皮石斛 GDP-甘露糖转运蛋白基因（DoGMT）在植物生长发育过程中甘露聚糖的生物合成中起关键作用。
Plant Sci. 2018 Dec;277:43-54. doi: 10.1016/j.plantsci.2018.07.021. Epub 2018 Sep 13.
8
Transport of UDP-galactose in plants. Identification and functional characterization of AtUTr1, an Arabidopsis thaliana UDP-galactos/UDP-glucose transporter.植物中UDP-半乳糖的转运。拟南芥UDP-半乳糖/UDP-葡萄糖转运蛋白AtUTr1的鉴定与功能表征
J Biol Chem. 2002 Sep 6;277(36):32923-9. doi: 10.1074/jbc.M204081200. Epub 2002 May 31.
9
Functional YFP-tagging of the essential GDP-mannose transporter reveals an important role for the secretion related small GTPase SrgC protein in maintenance of Golgi bodies in Aspergillus niger.对必需 GDP-甘露糖转运蛋白进行功能性 YFP 标记，揭示了分泌相关小分子 GTP 酶 SrgC 蛋白在黑曲霉高尔基体维持中的重要作用。
Fungal Biol. 2011 Mar;115(3):253-64. doi: 10.1016/j.funbio.2010.12.010. Epub 2010 Dec 29.
10
The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development.拟南芥的高尔基定位 GDP-L-岩藻糖转运蛋白对于植物发育是必需的。
Nat Commun. 2016 Jul 6;7:12119. doi: 10.1038/ncomms12119.
引用本文的文献
1
Genetic Editing of Promotes Immunity Against .对……的基因编辑可促进针对……的免疫。 （你提供的原文不完整，这是根据现有内容尽量完整翻译的结果 ）
Genes (Basel). 2025 Apr 21;16(4):470. doi: 10.3390/genes16040470.
2
Plays an Important Role in Growth, Reproduction, Virulence and Deoxynivalenol Biosynthesis of .在……的生长、繁殖、毒力和脱氧雪腐镰刀菌烯醇生物合成中起重要作用。 （原句表述不完整，缺少具体对象）
J Fungi (Basel). 2024 Mar 11;10(3):208. doi: 10.3390/jof10030208.
3
The ascorbate biosynthesis pathway in plants is known, but there is a way to go with understanding control and functions.植物中抗坏血酸的生物合成途径已为人所知，但在理解其调控和功能方面仍有很长的路要走。
J Exp Bot. 2024 May 3;75(9):2604-2630. doi: 10.1093/jxb/erad505.
4
Comparative transcriptome analysis reveals key pathways and genes involved in trichome development in tea plant ().比较转录组分析揭示了茶树毛状体发育过程中的关键途径和基因。
Front Plant Sci. 2022 Sep 23;13:997778. doi: 10.3389/fpls.2022.997778. eCollection 2022.
5
Systematic in silico discovery of novel solute carrier-like proteins from proteomes.系统计算发现蛋白质组中的新型溶质载体样蛋白。
PLoS One. 2022 Jul 28;17(7):e0271062. doi: 10.1371/journal.pone.0271062. eCollection 2022.
6
Two galacturonosyltransferases function in plant growth, stomatal development, and dynamics.两种半乳糖基转移酶在植物生长、气孔发育和动态中发挥作用。
Plant Physiol. 2021 Dec 4;187(4):2820-2836. doi: 10.1093/plphys/kiab432.
7
Biosynthesis and Transport of Nucleotide Sugars for Plant Hemicellulose.植物半纤维素的核苷酸糖生物合成与转运
Front Plant Sci. 2021 Nov 12;12:723128. doi: 10.3389/fpls.2021.723128. eCollection 2021.
8
The nucleotide sugar transporter GONST2 is a functional homolog of GONST1.核苷酸糖转运蛋白GONST2是GONST1的功能同源物。
Plant Direct. 2021 Mar 19;5(3):e00309. doi: 10.1002/pld3.309. eCollection 2021 Mar.
9
The Suitability of Orthogonal Hosts to Study Plant Cell Wall Biosynthesis.用于研究植物细胞壁生物合成的正交宿主的适用性
Plants (Basel). 2019 Nov 17;8(11):516. doi: 10.3390/plants8110516.
10
Evolution of the Cell Wall Gene Families of Grasses.禾本科植物细胞壁基因家族的进化
Front Plant Sci. 2019 Oct 4;10:1205. doi: 10.3389/fpls.2019.01205. eCollection 2019.
本文引用的文献
1
G.A.T.T. (A General Agreement on Traffic and Transport) and Brefeldin A in Plant Cells.《关贸总协定》（交通与运输总协定）与植物细胞中的布雷菲德菌素A
Plant Cell. 1994 Apr;6(4):463-467. doi: 10.1105/tpc.6.4.463.
2
Evidence for a UDP-Glucose Transporter in Golgi Apparatus-Derived Vesicles from Pea and Its Possible Role in Polysaccharide Biosynthesis.豌豆高尔基体衍生囊泡中UDP-葡萄糖转运蛋白的证据及其在多糖生物合成中的可能作用。
Plant Physiol. 1996 Dec;112(4):1585-1594. doi: 10.1104/pp.112.4.1585.
3
Brefeldin A Effects in Plants (Are Different Golgi Responses Caused by Different Sites of Action?).布雷菲德菌素A对植物的影响（不同作用位点是否会引发不同的高尔基体反应？）
Plant Physiol. 1997 Jun;114(2):401-403. doi: 10.1104/pp.114.2.401.
4
The ins(ide) and out(side) of dolichyl phosphate biosynthesis and recycling in the endoplasmic reticulum.内质网中磷酸多萜醇生物合成与循环的内部和外部情况
Glycobiology. 2001 May;11(5):61R-70R. doi: 10.1093/glycob/11.5.61r.
5
The gene defective in leukocyte adhesion deficiency II encodes a putative GDP-fucose transporter.白细胞黏附缺陷II型中的缺陷基因编码一种假定的GDP岩藻糖转运蛋白。
Nat Genet. 2001 May;28(1):69-72. doi: 10.1038/ng0501-69.
6
Plant glycosyltransferases.植物糖基转移酶
Curr Opin Plant Biol. 2001 Jun;4(3):219-24. doi: 10.1016/s1369-5266(00)00164-3.
7
SQV-7, a protein involved in Caenorhabditis elegans epithelial invagination and early embryogenesis, transports UDP-glucuronic acid, UDP-N- acetylgalactosamine, and UDP-galactose.SQV-7是一种参与秀丽隐杆线虫上皮内陷和早期胚胎发育的蛋白质，它能转运尿苷二磷酸葡萄糖醛酸、尿苷二磷酸-N-乙酰半乳糖胺和尿苷二磷酸半乳糖。
Proc Natl Acad Sci U S A. 2001 Mar 27;98(7):3738-43. doi: 10.1073/pnas.061593098. Epub 2001 Mar 20.
8
How the cell wall acquired a cellular context.细胞壁是如何获得细胞环境的。
Plant Physiol. 2001 Jan;125(1):127-30. doi: 10.1104/pp.125.1.127.
9
A proteomic analysis of organelles from Arabidopsis thaliana.拟南芥细胞器的蛋白质组学分析。
Electrophoresis. 2000 Oct;21(16):3488-99. doi: 10.1002/1522-2683(20001001)21:16<3488::AID-ELPS3488>3.0.CO;2-3.
10
Identification of a conserved motif in the yeast golgi GDP-mannose transporter required for binding to nucleotide sugar.鉴定酵母高尔基体GDP-甘露糖转运蛋白中与核苷酸糖结合所需的保守基序。
J Biol Chem. 2001 Feb 9;276(6):4424-32. doi: 10.1074/jbc.M009114200. Epub 2000 Nov 6.
Zotero 插件