Baldwin T C, Handford M G, Yuseff M I, Orellana A, Dupree P
Department of Biochemistry, University of Cambridge, Building O, Downing Site, Cambridge CB2 1QW, United Kingdom.
Plant Cell. 2001 Oct;13(10):2283-95. doi: 10.1105/tpc.010247.
Transport of nucleotide sugars across the Golgi apparatus membrane is required for the luminal synthesis of a variety of plant cell surface components. We identified an Arabidopsis gene encoding a nucleotide sugar transporter (designated GONST1) that we have shown by transient gene expression to be localized to the Golgi. GONST1 complemented a GDP-mannose transport-defective yeast mutant (vrg4-2), and Golgi-rich vesicles from the complemented strain displayed increased GDP-mannose transport activity. GONST1 promoter::beta-glucuronidase studies suggested that this gene is expressed ubiquitously. The identification of a Golgi-localized nucleotide sugar transporter from plants will allow the study of the importance of this class of proteins in the synthesis of plant cell surface components such as cell wall polysaccharides.
多种植物细胞表面成分的腔内合成需要核苷酸糖跨高尔基体膜运输。我们鉴定出拟南芥中一个编码核苷酸糖转运蛋白的基因（命名为GONST1），通过瞬时基因表达我们已证明它定位于高尔基体。GONST1弥补了GDP-甘露糖转运缺陷型酵母突变体（vrg4-2），并且来自互补菌株的富含高尔基体的囊泡显示出增加的GDP-甘露糖转运活性。GONST1启动子::β-葡萄糖醛酸酶研究表明该基因在植物中普遍表达。从植物中鉴定出一种定位于高尔基体的核苷酸糖转运蛋白，将有助于研究这类蛋白质在植物细胞表面成分（如细胞壁多糖）合成中的重要性。
