Armstrong C, Hawkes R
Department of Cell Biology and Anatomy and Genes and Development Research Group, Faculty of Medicine, The University of Calgary, Calgary, Alberta T2N 4N1, Canada.
J Comp Neurol. 2001 Oct 15;439(2):151-61. doi: 10.1002/cne.1339.
The adult mouse cerebellar vermis consists of four transverse zones, each of which is further subdivided into parasagittal stripes. In the adult weaver (wv/wv) mouse, the zebrin II expression pattern in the cerebellar vermis is abnormal, consistent with the absence of a central zone (approximately lobules VI/VII). Because the small, heat shock protein HSP25 is a constitutive marker of parasagittal bands of Purkinje cells in the caudal central zone and the nodular zone (approximately lobules IX/X), we used HSP25 immunocytochemistry to show that the patterning abnormalities in wv/wv reflect selective Purkinje cell ectopia rather than the absence of the central zone. A specific HSP25-immunopositive Purkinje cell ectopia within the central zone was identified. Symmetrical clusters of HSP25-immunopositive Purkinje cells, which presumably would have formed the parasagittal stripes in the wild type, are present ectopically on either side of the midline in wv/wv. In contrast, in the nodular zone, HSP25-immunopositive Purkinje cells form a near-monolayer and are organized into parasagittal stripes. We therefore conclude that specific Purkinje cell clusters in the wv/wv cerebellum fail to disperse and that this ectopia contributes to the topographical abnormalities.
成年小鼠的小脑蚓部由四个横向区域组成，每个区域又进一步细分为矢状旁条纹。在成年织工（wv/wv）小鼠中，小脑蚓部的zebrin II表达模式异常，这与中央区域（大约小叶VI/VII）的缺失一致。由于小热休克蛋白HSP25是尾侧中央区域和小结区域（大约小叶IX/X）浦肯野细胞矢状旁带的组成性标记，我们使用HSP25免疫细胞化学来表明wv/wv中的模式异常反映了选择性浦肯野细胞异位，而不是中央区域的缺失。在中央区域内鉴定出了特定的HSP25免疫阳性浦肯野细胞异位。HSP25免疫阳性浦肯野细胞的对称簇，在野生型中大概会形成矢状旁条纹，在wv/wv中异位地出现在中线两侧。相反，在小结区域，HSP25免疫阳性浦肯野细胞形成近乎单层并组织成矢状旁条纹。因此，我们得出结论：wv/wv小脑中特定的浦肯野细胞簇未能分散，并且这种异位导致了地形异常。
