Armstrong C L, Chung S-H, Armstrong J N, Hochgeschwender U, Jeong Y-G, Hawkes R
Department of Biomedical Science, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
J Comp Neurol. 2009 Dec 1;517(4):524-38. doi: 10.1002/cne.22167.
Somatostatin 28 immunoreactivity (Sst28-ir) identifies a specific subset of mossy fiber terminals in the adult mouse cerebellum. By using double-labeling immunohistochemistry, we determined that Sst28-ir is associated with presynaptic mossy fiber terminal rosettes, and not Purkinje cells, Golgi cells, or unipolar brush cells. Sst28-ir mossy fibers are restricted to the central zone (lobules VI/VII) and nodular zone (lobules IX, X) of the vermis, and the paraflocculus and flocculus. Within each transverse zone the mossy fiber terminal fields form a reproducible array of parasagittal stripes. The boundaries of Sst28-ir stripes align with a specific array of Purkinje cell stripes revealed by using immunocytochemistry for the small heat shock protein HSP25. In the cerebellum of the homozygous weaver mouse, in which a subpopulation of HSP25-ir Purkinje cells are located ectopically, the corresponding Sst28-ir mossy fiber projection is also ectopic, suggesting a role for a specific Purkinje cell subset in afferent pattern formation. Likewise, in the scrambler mutant mouse, Sst28-ir mossy fibers show a very close association with HSP25-ir Purkinje cell clusters. HSP25 itself does not appear to be critical for normal patterning, however: in the KJR mouse, which does not express cerebellar HSP25, Sst28 expression appears to be normal. Likewise, the Purkinje cell patterning antigens zebrin II and HSP25 are expressed normally in both Sst- and Sst-receptor knockout mice, suggesting that somatostatinergic transmission is not necessary for Purkinje cell stripe formation.
生长抑素28免疫反应性（Sst28-ir）可识别成年小鼠小脑中苔藓纤维终末的一个特定亚群。通过使用双标免疫组织化学方法，我们确定Sst28-ir与突触前苔藓纤维终末玫瑰花结相关，而非浦肯野细胞、高尔基细胞或单极刷细胞。Sst28-ir苔藓纤维局限于蚓部的中央区（小叶VI/VII）和小结区（小叶IX、X）以及旁绒球和绒球。在每个横向区域内，苔藓纤维终末场形成可重复的矢状旁条纹阵列。Sst28-ir条纹的边界与通过对小分子热休克蛋白HSP25进行免疫细胞化学显示的特定浦肯野细胞条纹阵列对齐。在纯合weaver小鼠的小脑中，其中一部分HSP25-ir浦肯野细胞异位定位，相应的Sst28-ir苔藓纤维投射也异位，提示特定浦肯野细胞亚群在传入模式形成中起作用。同样，在scrambler突变小鼠中，Sst28-ir苔藓纤维与HSP25-ir浦肯野细胞簇显示出非常紧密的关联。然而，HSP25本身似乎对正常模式形成并不关键：在不表达小脑HSP25的KJR小鼠中，Sst28表达似乎正常。同样，浦肯野细胞模式形成抗原zebrin II和HSP25在Sst和Sst受体敲除小鼠中均正常表达，表明生长抑素能传递对于浦肯野细胞条纹形成并非必需。
