Garkavenko O, Obriadina A, Meng J, Anderson D A, Benard H J, Schroeder B A, Khudyakov Y E, Fields H A, Croxson M C
Virology Laboratory, Diatranz Ltd., PO Box 23566, Hunters Corner, Auckland, New Zealand.
J Med Virol. 2001 Nov;65(3):525-9.
The objectives of the present study were to establish the presence of hepatitis E virus (HEV) in New Zealand pigs, first by testing for HEV antibody in pig herds throughout New Zealand to measure the herd prevalence, then by attempting to amplify HEV genomic sequences by PCR. Antibody was measured by two independently designed ELISA serology tests. HEV RNA fragments were amplified by RT-PCR of nucleic acid extracted from faeces of 10-12-week-old piglets using primers targeting ORF1, ORF2, and ORF2/3. PCR products were subject to phylogenetic analysis. Antibody to HEV was found throughout New Zealand pig herds as well as in the different age groups within the herds. Twenty herds from 22 tested were positive for HEV antibody (91% herd prevalence). Phylogenetic analysis of the amplified sequences placed this New Zealand strain of HEV closest to the human European strain It-1 (AF 110390) and U.S. swine strain (AF 082843) with 88% and 83% similarity respectively in ORF1. It was concluded that HEV is widely distributed in the New Zealand pig population. Phylogenetic analysis shows that this is a new HEV strain, grouping most closely with the United States/European cluster, which includes HEV strains of both human and swine origin.
本研究的目的是确定新西兰猪中戊型肝炎病毒（HEV）的存在情况，首先通过检测新西兰各地猪群中的HEV抗体来衡量群体流行率，然后尝试通过PCR扩增HEV基因组序列。抗体通过两种独立设计的ELISA血清学检测进行测定。使用靶向ORF1、ORF2和ORF2/3的引物，通过RT-PCR对10至12周龄仔猪粪便中提取的核酸进行扩增，以扩增HEV RNA片段。PCR产物进行系统发育分析。在新西兰各地的猪群以及猪群中的不同年龄组中均发现了HEV抗体。在22个检测的猪群中，有20个猪群的HEV抗体呈阳性（群体流行率为91%）。对扩增序列的系统发育分析表明，这种新西兰的HEV毒株与人类欧洲毒株It-1（AF 110390）和美国猪毒株（AF 082843）最为接近，在ORF1中的相似性分别为88%和83%。得出的结论是，HEV在新西兰猪群中广泛分布。系统发育分析表明，这是一种新的HEV毒株，与包括人类和猪源HEV毒株的美国/欧洲簇最为紧密地聚类。
