Munné María S, Vladimirsky Sara, Otegui Lucio, Castro Raúl, Brajterman Leonardo, Soto Sonia, Guarnera Eduardo, Molina Viviana, Monfellano Marcela, Schlauder George G, González Jorge E
Nacional Reference Lab for Viral Hepatitis, National Institute of Infectious Diseases, ANLIS "Dr C.G. Malbrán", Buenos Aires, Argentina.
J Med Virol. 2006 Dec;78(12):1579-83. doi: 10.1002/jmv.20741.
In Argentina, a country considered non-endemic for hepatitis E virus (HEV) infection, serologic evidence of HEV infection has been observed in different human population groups. In other countries, a high degree of genetic relatedness has been observed between human and swine HEV genotype 3 sequences, suggesting zoonosis as one probable route of infection. This is the first identification of swine HEV in South America. HEV RNA was detected and sequenced in the ORF 1 and ORF 2 regions from swine fecal samples from a herd located in Pergamino, in the province of Buenos Aires. These strains all group into genotype 3 and exhibit a close relationship to two novel HEV variants previously identified in Argentina from sporadic acute cases of non-A to -C hepatitis in humans. In addition, using a modified commercial ELISA, the presence of anti-HEV antibodies was surveyed in five provinces across the country and all five showed a prevalence of HEV antibodies, ranging from 4% to 58%. The results suggest that swine could be an important reservoir for virus transmission in Argentina as has been suggested for other non-endemic areas. The Argentine human strains and swine strain described in this article seem to be closely related to a human Austrian strain, suggesting a potential European origin of HEV infection in these cases.
在阿根廷这个被认为并非戊型肝炎病毒（HEV）感染地方性流行的国家，已在不同人群中观察到HEV感染的血清学证据。在其他国家，已观察到人类和猪的HEV 3型序列之间存在高度的遗传相关性，这表明人畜共患病是一种可能的感染途径。这是在南美洲首次鉴定出猪HEV。从位于布宜诺斯艾利斯省佩尔加米诺的一个猪群的粪便样本中，在开放阅读框1（ORF 1）和开放阅读框2（ORF 2）区域检测到HEV RNA并进行了测序。这些毒株均归为3型，并且与之前在阿根廷从散发的非甲至非丙型肝炎急性病例中鉴定出的两种新型HEV变异体关系密切。此外，使用改良的商业酶联免疫吸附测定法（ELISA），对全国五个省份进行了抗HEV抗体检测，所有五个省份均显示出HEV抗体的流行率，范围从4%至58%。结果表明，猪可能是阿根廷病毒传播的重要宿主，就像在其他非地方性流行地区所表明的那样。本文中描述的阿根廷人类毒株和猪毒株似乎与一株奥地利人类毒株密切相关，这表明在这些病例中HEV感染可能起源于欧洲。
