Chen Li-li, Sun Wei-lian, Yan Jie, Yu Zhong-sheng
Department of Stomatology, The Second Affiliated Hospital, College of Medical Sciences, Zhejiang University, Hangzhou 310009, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2006 Apr;41(4):212-5.
To investigate the correlation between infection of different human cytomegalovirus (HCMV) glycoprotein B (gB) genotypes and human chronic periodontitis.
A nested-polymerase chain reaction (nPCR) was employed to detect HCMV gB gene in the subgingival plaque samples from 65 chronic periodontitis patients and in the gingival crevicular fluid samples from 24 periodontally healthy control. The amplification fragments of gB gene were further genotyped by restriction fragment length polymorphism (RFLP). The correlation among infection with the different HCMV genotypes and the severity of periodontal lesion were evaluated.
In terms of teeth examined, the prevalence of HCMV (59.23%, 154/260) in the chronic periodontitis lesions was significantly higher than that of HCMV (32.29%, 31/96) in the periodontally healthy control (P < 0.01). Of the HCMV DNA positive samples from the chronic periodontitis lesions, 11.7% (18/154) was genotyped as gB I, 80.5% (124/154) as gB II, and 7.8% (12/154) as gB I and gB II co-infection, and of the HCMV DNA positive samples from the periodontal healthy control, 45.2% (14/31) was genotyped as gB I, 38.7% (12/31) as gB II, and 16.1% (5/31) as gB I and gB II co-infection. The gB II genotype was more dominant among the chronic periodontitis lesions compared with that among the periodontally healthy control (P < 0.01). In chronic periodontitis, no statistical significance could be found between infection of different HCMV gB genotypes and the different clinical parameters of CAL, PD and GI (P > 0.05).
Subgingival infection with HCMV is closely associated with chronic periodontitis. Infection of HCMV may not correlate directly with severity of periodontitis. However, gB II may be the dominant genotype of HCMV, which is associated with the chronic periodontitis.
探讨不同人类巨细胞病毒（HCMV）糖蛋白B（gB）基因型感染与人类慢性牙周炎之间的相关性。
采用巢式聚合酶链反应（nPCR）检测65例慢性牙周炎患者龈下菌斑样本及24例牙周健康对照者龈沟液样本中的HCMV gB基因。gB基因扩增片段进一步通过限制性片段长度多态性（RFLP）进行基因分型。评估不同HCMV基因型感染与牙周病变严重程度之间的相关性。
就检查的牙齿而言，慢性牙周炎病变中HCMV的患病率（59.23%，154/260）显著高于牙周健康对照中HCMV的患病率（32.29%，31/96）（P<0.01）。慢性牙周炎病变中HCMV DNA阳性样本中，11.7%（18/154）基因分型为gB I，80.5%（124/154）为gB II，7.8%（12/154）为gB I和gB II共感染；牙周健康对照中HCMV DNA阳性样本中，45.2%（14/31）基因分型为gB I，38.7%（12/31）为gB II，16.1%（5/31）为gB I和gB II共感染。与牙周健康对照相比，gB II基因型在慢性牙周炎病变中更占优势（P<0.01）。在慢性牙周炎中，不同HCMV gB基因型感染与临床附着丧失（CAL）、牙周袋深度（PD）和牙龈指数（GI）的不同参数之间未发现统计学意义（P>0.05）。
龈下HCMV感染与慢性牙周炎密切相关。HCMV感染可能与牙周炎严重程度无直接关联。然而，gB II可能是与慢性牙周炎相关的HCMV优势基因型。
