Przybecki Z, Olejniczak J, Adamska E
Department of Plant Genetics, Breeding and Plant Biotechnology, Warsaw University of Agriculture, Nowoursynowska 166, 02-787 Warszawa, Poland.
Cell Mol Biol Lett. 2001;6(3):587-91.
In order to regenerate Cuphea tolucana from hypocotyl, cotyledon and root explants, a solid culture and 8 hormone combinations were used. Only the root explants did not react to any of the media. On most of the media, the other explants formed shoots, roots or callus, or their reaction was more complex. The regeneration probably occurred via direct organogenesis. The regenerants displayed a wide variety of morphological characteristics. However, their offspring did not show any differences from plants, which had not undergone culture.
为了从萼距花的下胚轴、子叶和根外植体再生植株,采用了固体培养和8种激素组合。只有根外植体对任何一种培养基都没有反应。在大多数培养基上,其他外植体形成了芽、根或愈伤组织,或者它们的反应更为复杂。再生可能通过直接器官发生进行。再生植株表现出各种各样的形态特征。然而,它们的后代与未经过培养的植株没有任何差异。