Dovzhenko Alexander, Koop Hans-Ulrich
Faculty of Biology, Department I, Botany, Ludwig-Maximilans-Universität, Menzinger Str. 67, 80638 Munich, Germany.
Planta. 2003 Jul;217(3):374-81. doi: 10.1007/s00425-003-1006-7. Epub 2003 Mar 14.
The successful application of recombinant DNA technology for crop plants requires efficient regeneration systems. A detailed study on the regeneration potential of callus and callus-derived protoplasts of a recalcitrant species, sugarbeet, was performed. A reproducible and highly efficient method for induction of regenerable friable callus was established from etiolated hypocotyl explants. A reduced sucrose concentration proved beneficial. Successful shoot regeneration could be demonstrated in 10 out of 12 tested lines. Seed germination, followed by callus induction and shoot regeneration required only a single culture medium. Additionally, the regeneration capacity of roots and root-derived callus was demonstrated. Highly efficient plant regeneration was also achieved when using protoplasts isolated from regenerable friable callus induced on etiolated hypocotyls explants. To our knowledge this represents the first report on callus protoplast to plant regeneration in sugarbeet.
重组DNA技术在农作物上的成功应用需要高效的再生系统。我们对一种顽拗型植物——甜菜的愈伤组织和愈伤组织来源的原生质体的再生潜力进行了详细研究。从黄化下胚轴外植体建立了一种可重复且高效的诱导可再生易碎愈伤组织的方法。降低蔗糖浓度被证明是有益的。在12个测试品系中，有10个品系成功实现了芽再生。种子萌发，随后的愈伤组织诱导和芽再生仅需一种培养基。此外，还证明了根和根来源的愈伤组织的再生能力。当使用从黄化下胚轴外植体诱导产生的可再生易碎愈伤组织中分离的原生质体时，也实现了高效的植株再生。据我们所知，这是关于甜菜愈伤组织原生质体再生植株的首次报道。
