Fan Xiao-feng, Yang Ying-li, Guo Xiao-qiang, Liu Xiu-li
Department of Life Science, Longdong University, Qingyang 745000, China.
Zhong Yao Cai. 2009 Feb;32(2):173-6.
To investigate the influential factors and establish culture method for G. uralensis callus.
To study the possible effective factors of culture condition by comparing with different explants, light, plant hormones and its ratio.
The hypocotyl was the best among different explants, its inducing ratios was 94%, and the callus occurred earliest. When the calluses were inoculated on MS + 6 - BA (1.0-2.0) mg/L + NAA (0.5-1.0) mg/L, adventitious buds formed. 2,4-D could induce non-embryogenic callus, the compounding proportions of 6 - BA and NAA could induce embryogenic callus. Light influenced induction and growth of callus.
Different explants, components of hormone and light are the influencial factors of callus induction and growth of G. uralensis.
探讨影响甘草愈伤组织诱导的因素并建立其培养方法。
通过比较不同外植体、光照、植物激素及其比例，研究培养条件的可能有效因素。
在不同外植体中，下胚轴最佳，诱导率为94%，且愈伤组织形成最早。将愈伤组织接种于MS + 6 - BA（1.0 - 2.0）mg/L + NAA（0.5 - 1.0）mg/L上时，不定芽形成。2,4 - D可诱导非胚性愈伤组织，6 - BA与NAA的配比可诱导胚性愈伤组织。光照影响愈伤组织的诱导和生长。
不同外植体、激素成分及光照是影响甘草愈伤组织诱导和生长的因素。
