Webber M M, Stonington O G
J Urol. 1975 Aug;114(2):246-8. doi: 10.1016/s0022-5347(17)66998-2.
The primary objective of our study was to obtain pure cultures of prostatic epithelium. The phenomena of encapsulation by epithelial cells and hypocellularity in stroma occurred when explants of human prostatic tissue were maintained in suspension cultures. Hypocellularity progressed with time and was more pronounced in encapsulated explants. When encapsulated explants were allowed to attach to the substrate they formed an outgrowth of epithelial cells in a monolayer. The significance of these findings is in the use of the described changes in isolating and establishing epithelial cultures of human prostatic epithelium. These cultures are required for studies on the biology of prostatic epithelium and the etiology and treatment of prostatic neoplasia.
我们研究的主要目的是获得前列腺上皮的纯培养物。当人前列腺组织外植体维持在悬浮培养中时，会出现上皮细胞包囊化和基质细胞减少的现象。细胞减少随时间进展，在包囊化外植体中更明显。当包囊化外植体附着于基质时，它们形成单层上皮细胞的生长物。这些发现的意义在于利用所描述的变化来分离和建立人前列腺上皮的培养物。这些培养物对于前列腺上皮生物学以及前列腺肿瘤的病因学和治疗研究是必需的。
