Gorid'ko T M, Hula N M, Marhitych V M, Hovseieva N M, Klimashevs'kyĭ V M, Shahidulin M Iu
Palladin Institute of Biochemistry, NAS of Ukraine.
Ukr Biokhim Zh (1999). 2001 Jan-Feb;73(1):82-7.
The effect of N-palmitoylethanolamine (NPE, 10(-5) M) on the lipid peroxidation (LPO) processes, phospholipid and fatty acid content in the rat liver at perfusion and ischemia during the liver preservation was estimated. As early as at the 5th min of perfusion by cooled conserving solution "Eurocollins", LPO activation was determined. Simultaneously the content of lysophosphatidylcholine (LPC) and phosphatidylserine increased, the total cholesterol level decreased. The redistribution of saturated and unsaturated fatty acids quantity was detected. The addition of NPE into "Eurocollins" solution reduced the accumulation of malonedialdehyde and LPC, modified the fatty acids content. These effects, evidently, formed the basis of the protective action of NPE on the hepatic tissues under anoxia.
评估了N-棕榈酰乙醇胺（NPE，10⁻⁵ M）对大鼠肝脏在保存过程中灌注和缺血时脂质过氧化（LPO）过程、磷脂和脂肪酸含量的影响。早在用冷却的“Eurocollins”保存液灌注第5分钟时，就确定了LPO的激活。同时，溶血磷脂酰胆碱（LPC）和磷脂酰丝氨酸的含量增加，总胆固醇水平降低。检测到饱和脂肪酸和不饱和脂肪酸数量的重新分布。向“Eurocollins”溶液中添加NPE可减少丙二醛和LPC的积累，改变脂肪酸含量。显然，这些作用构成了NPE对缺氧状态下肝组织保护作用的基础。
