Gulaia N M, Babich L G, Shlykov S G, Margitich V M, Govseeva N N, Klimashevskiĭ V M, Kosterin S A
A.V. Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv.
Ukr Biokhim Zh (1978). 1997 Sep-Dec;69(5-6):75-84.
N-palmitoylethanolamine (NPE) was studied for its effect on the systems of energy-dependent transport of Ca2+ in the intracell structures (mitochondria, endoplasmic reticulum) of permeabilized cells of smooth muscles of the rat uterus as well as on the lipid composition of myocytes. NPE in concentration of 10(-5) M partially (by 30-50%) inhibited energy-dependent accumulation of Ca2+ in endoplasmic reticulum and mitochondria of myometrium cells permeabilized by means of treatment of myocytes suspension by digitonin (0.1 mg/ml). NPE modifies the lipid composition of permeabilized myocytes when determining the increase of the amount of inorganic phosphorus of total phospholipids by 57.3% at the expense of considerable accumulation of phosphatidylcholine, phosphatidylethanolamine and sphingomyelin. It is supposed that the effect of NPE on the systems of energy-dependent transport of Ca2+ is achieved through the modification of phospholipid composition of a cell, while modulating effect of NPE on the active transmembrane transfer of Ca2+ in the intracellular structure can be an important link in the general mechanism of the effect of this compound on Ca2+ metabolism in myometrium and on Ca(2+)-depended control of the contracting function of the uterus.
研究了N-棕榈酰乙醇胺（NPE）对大鼠子宫平滑肌通透细胞内细胞器（线粒体、内质网）中Ca2+能量依赖性转运系统以及对心肌细胞脂质组成的影响。浓度为10^(-5) M的NPE部分（30%-50%）抑制了通过用洋地黄皂苷（0.1 mg/ml）处理心肌细胞悬液而通透的子宫肌层细胞内质网和线粒体中Ca2+的能量依赖性积累。在测定总磷脂中无机磷含量增加57.3%时，NPE改变了通透心肌细胞的脂质组成，这是以磷脂酰胆碱、磷脂酰乙醇胺和鞘磷脂的大量积累为代价的。据推测，NPE对Ca2+能量依赖性转运系统的作用是通过改变细胞的磷脂组成实现的，而NPE对细胞内结构中Ca2+主动跨膜转运的调节作用可能是该化合物对子宫肌层Ca2+代谢以及对子宫收缩功能的Ca(2+)依赖性控制作用的一般机制中的一个重要环节。
