Gulaia N M, Govseeva N N, Klimashevskiĭ V M, Shinlova O P, Slinchenko N M, Margitich V M, Kosterin S A
A.V. Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv.
Ukr Biokhim Zh (1978). 1997 Sep-Dec;69(5-6):64-74.
N-palmitoylethanolamine (NPE) was studied for their effect on calcium pump of pig myometrium sarcolemma. NPE in concentration of 10 microM, stimulated by 28-46% Mg2+, ATP-dependent accumulation of Ca2+ in vesicles of plasmatic membrane of uterus myocytes taking absolutely no effect on passive release of this cation from them. NPE modified phospholipid composition of sarcolemma, causing the increase of percentage content of phosphatidylinositol (by 20.2%) and lysophosphatidylcholine (2.7 times). While NPE effects transport Ca2+, Mg(2+)-ATPase solubilized from plasmatic membrane and purified due to the method of affinity chromatography on calmodulin-sepharose 4B, no activating effect of NPE on the calcium pump was observed. And what is more, a weakly expressed tendency to inhibition (by 14-15%, respectively) of the rate of Ca2+, Mg(2+)-dependent enzymic hydrolysis of ATP has been revealed. It is supposed that the effect of NPE on active transmembrane transport of Ca2+ is an important link in the general mechanism of contraction-relax of the myometrium and is, apparently, connected with its modifying effect on the lipid composition of the sarcolemma.
研究了N-棕榈酰乙醇胺（NPE）对猪子宫肌层肌膜钙泵的影响。浓度为10微摩尔的NPE，在28 - 46%的Mg2+刺激下，子宫肌细胞质膜囊泡中Ca2+的ATP依赖性积累增加，对该阳离子从囊泡中的被动释放没有影响。NPE改变了肌膜的磷脂组成，导致磷脂酰肌醇的百分比含量增加（增加20.2%）以及溶血磷脂酰胆碱增加（增加2.7倍）。虽然NPE影响从质膜溶解并通过钙调蛋白-琼脂糖4B亲和层析法纯化的Ca2+、Mg(2+)-ATP酶的转运，但未观察到NPE对钙泵有激活作用。此外，还发现了一种弱表达的趋势，即Ca2+、Mg(2+)-依赖性ATP酶促水解速率分别被抑制14 - 15%。据推测，NPE对Ca2+的主动跨膜转运的影响是子宫肌层收缩-舒张一般机制中的一个重要环节，并且显然与其对肌膜脂质组成的修饰作用有关。
