Savagner F, Franc B, Guyetant S, Rodien P, Reynier P, Malthiery Y
Inserm EMI-U 00-18, Laboratoire de Biochimie et Biologie Moléculaire, 4rue Larrey, F-49033 Angers cedex 01, France.
J Clin Endocrinol Metab. 2001 Oct;86(10):4920-5. doi: 10.1210/jcem.86.10.7894.
Oxyphilic tumors (oncocytomas or Hürthle cell tumors) form a rare subgroup of thyroid tumors characterized by cells containing abundant mitochondria. The relationship between the mitochondrial proliferation and the pathogenesis of these tumors is unknown. We have assessed the expression of the mitochondrial ND2 and ND5 (subunits of the nicotinamide adenine dinucleotide dehydrogenase complex) genes and the nuclear UCP2 (uncoupling protein 2) gene in 22 oxyphilic thyroid tumors and matched controls. The consumption of oxygen in mitochondria from tumors was determined by polarography. ATP assays were used to explore the mitochondrial respiratory chain activity and the oxidative phosphorylation coupling in seven fresh thyroid tumors and controls. Adenosine triphosphate synthesis was significantly lower in all the tumors, compared with controls, suggesting that a coupling defect in oxidative phosphorylation may be a cause of mitochondrial hyperplasia in oxyphilic thyroid tumors.
嗜酸性肿瘤（嗜酸细胞瘤或许特耳细胞肿瘤）是甲状腺肿瘤中一个罕见的亚组，其特征是细胞内含有丰富的线粒体。线粒体增殖与这些肿瘤发病机制之间的关系尚不清楚。我们评估了22例嗜酸性甲状腺肿瘤及其配对对照中线粒体ND2和ND5（烟酰胺腺嘌呤二核苷酸脱氢酶复合物的亚基）基因以及核UCP2（解偶联蛋白2）基因的表达。通过极谱法测定肿瘤线粒体中的氧气消耗。采用ATP检测法探究7例新鲜甲状腺肿瘤及其对照中线粒体呼吸链活性和氧化磷酸化偶联情况。与对照相比，所有肿瘤中的三磷酸腺苷合成均显著降低，这表明氧化磷酸化的偶联缺陷可能是嗜酸性甲状腺肿瘤中线粒体增生的一个原因。
