Stankov Karmen, Biondi Annalisa, D'Aurelio Marilena, Gasparre Giuseppe, Falasca Anna, Romeo Giovanni, Lenaz Giorgio
Dipartimento di Medicina Interna, Cardioangiologia ed Epatologia, Unita' Operativa di Genetica Medica, Policlinico S. Orsola-Malpighi, Bologna, Italy.
Thyroid. 2006 Apr;16(4):325-31. doi: 10.1089/thy.2006.16.325.
We conducted a study on the functional characteristics of mitochondria in an oxyphilic thyroid tumor cell line, which may provide useful clues about Hürthle cell tumors carcinogenesis.
The functional study on thyroid tumors with cell oxyphilia (Hürthle cell tumors), characterized by mitochondrial hyperplasia, was carried out in XTC.UC1, and B-CPAP, an oxyphilic and nonoxyphilic thyroid tumor cell line, respectively.
XTC.UC1 cell line showed higher activity of mitochondrial respiratory complexes I and II and decreased activity of complex III. The increased activity of complex I was not matched by increased expression of complex I subunits. The XTC.UC1 cells relied mostly on oxidative phosphorylation for energy conservation, although their mitochondrial energetic function was less efficient when related to mitochondrial content of the cells. Finally, the oxyphilic cell line produced significantly higher amounts of reactive oxygen species (ROS) in comparison with B-CPAP cell line.
The involvement of ROS in mitochondrial biogenesis and proliferation as well as in carcinogenesis and apoptosis indicate that differences in activity of respiratory chain components and their unbalance may be responsible for development of morphological and functional changes observed in thyroid tumors with cell oxyphilia.
我们对嗜酸性甲状腺肿瘤细胞系中线粒体的功能特性进行了研究，这可能为许特莱细胞肿瘤的致癌机制提供有用线索。
分别在XTC.UC1（一种嗜酸性甲状腺肿瘤细胞系）和B-CPAP（一种嗜酸性及非嗜酸性甲状腺肿瘤细胞系）中对以线粒体增生为特征的嗜酸性甲状腺肿瘤（许特莱细胞肿瘤）进行功能研究。
XTC.UC1细胞系显示线粒体呼吸复合体I和II的活性较高，而复合体III的活性降低。复合体I活性的增加与其亚基表达的增加不匹配。XTC.UC1细胞主要依靠氧化磷酸化来保存能量，尽管与细胞的线粒体含量相关时其线粒体能量功能效率较低。最后，与B-CPAP细胞系相比，嗜酸性细胞系产生的活性氧（ROS）显著更多。
ROS参与线粒体生物发生和增殖以及致癌作用和细胞凋亡，这表明呼吸链成分活性的差异及其失衡可能是嗜酸性甲状腺肿瘤中观察到的形态和功能变化发生的原因。
