[Soft extravascular model (SEM) with beta-radioactive ray inhibits smooth muscle cell proliferation and intimal hyperplasia in autogenous vein graft].

OBJECTIVE

To investigate the effects of the soft extravascular model (SEM) with 32P on intimal hyperplasia and smooth muscle cell preliferation in autogenous vein grafts.

METHODS

We transplanted the femoral veins to the co-lateral femoral arteries in rabbits. The animals were divided into three groups of Aspirin group, 32P group and control group. We observed the expression density of endothelin type A receptor cDNA (ETAcDNA) and intimal relative thickness by using in situ molecular hybridization of ETAcDNA by computerized image analysis technology.

RESULTS

SEM with beta-ray obviously inhibited SMC preliferation from 1 W to 12 W postoperation, and notably restrained intimal hyperplasia at early to middle stage of graft. Aspirin could not inhibit SMC proliferation and intimal hyperplasia under the condition of this experiment.