Jamal S, Picker L J, Aquino D B, McKenna R W, Dawson D B, Kroft S H
University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390-9073, USA.
Am J Clin Pathol. 2001 Oct;116(4):512-26. doi: 10.1309/QF6N-VAQW-N74H-4JE2.
We retrospectively reviewed multiparameter flow cytometric analyses in 50 peripheral T-cell neoplasms (PTCNs). Results were interpreted within the context of a large cohort of nonneoplastic T-cell populations. All PTCN diagnoses were confirmed with morphologic and/or molecular analysis. Aberrant populations were defined as discrete immunophenotypic clusters exhibiting loss of or increased or diminished expression of T-cell antigens relative to internal immunophenotypically normal T-cell populations. An antigenic pattern was considered abnormal if it exceeded ranges for T-cell subsets in specific anatomic sites or was not normally encountered. Forty-six of 50 and 41 of 50 demonstrated 1 or more and 2 or more aberrations, respectively. The most common abnormally expressed antigen was CD3, followed by CD7, CD5, and CD2. Except for CD7, abnormally dim or bright antigen expression was more common than deletion. Only 3 cases were abnormal solely based on expansion of an otherwise immunophenotypically normal population; the remainder had patterns of antigen expression not seen in nonneoplastic populations. These data indicate that most PTCNs are aberrant by multiparameter flow analysis. However, results must be interpreted within the context of thorough knowledge of the immunophenotypic spectrum of nonneoplastic T cells.
我们回顾性分析了50例外周T细胞肿瘤（PTCN）的多参数流式细胞术分析结果。结果在大量非肿瘤性T细胞群体的背景下进行解读。所有PTCN诊断均通过形态学和/或分子分析得以证实。异常群体被定义为相对于内部免疫表型正常的T细胞群体，表现出T细胞抗原表达缺失、增加或减少的离散免疫表型簇。如果抗原模式超出特定解剖部位T细胞亚群的范围或未正常出现，则被认为是异常的。50例中有46例和50例中有41例分别表现出1种或更多种和2种或更多种异常。最常异常表达的抗原是CD3，其次是CD7、CD5和CD2。除CD7外，异常低表达或高表达抗原比缺失更为常见。仅3例仅基于免疫表型正常群体的扩增而异常；其余病例具有非肿瘤性群体中未见的抗原表达模式。这些数据表明，通过多参数流式分析，大多数PTCN是异常的。然而，结果必须在全面了解非肿瘤性T细胞免疫表型谱的背景下进行解读。
