[川芎嗪对免疫介导型再生障碍性贫血小鼠骨髓细胞黏附分子表达的影响研究]
[Study on the effect of ligustrazine on adherent molecule expression of bone marrow cells in immune-mediated aplastic anemia mice].
作者信息
Dong L, Liu W, Sun H
机构信息
Department of Internal Medicine, Tongji Hospital, Tongji Medical University, Wuhan 430030.
出版信息
Zhonghua Xue Ye Xue Za Zhi. 1999 Apr;20(4):178-9.
OBJECTIVE
To explore the effect of ligustrazine on the expression of adherent molecule of bone marrow cells in immune-mediated aplastic anemia(AA) mice.
METHODS
Each immune-mediated AA mouse was intraperitoneally injected with 5 mg ligustrazine twice a day. On the 14th day, expressions of CD49d and CD49c in bone marrow mononuclear cells(MNC) were detected by flow cytometry, and VCAM-1 on stromal cells by immunohistochemistry SABC.
RESULTS
Expressions of CD49d, CD49e and VCAM-1 were significantly lower in AA group than in normal group(t = 11.96, 17.18 and 7.09 respectively, P < 0.001), and were much higher in ligustrazine treated group than in non-treated group(t = 9.05, 14.61 and 6.91 respctively, P < 0.001). Expressions of CD49d and VCAM-1 were in the normal range in ligustrazine group.
CONCLUSIONS
Ligustrazine can increase the expression of adherent molecule of bone marrow cells in immune-mediated AA mice, thus promote the growth of hematopoietic cells.
目的
探讨川芎嗪对免疫介导型再生障碍性贫血（AA）小鼠骨髓细胞黏附分子表达的影响。
方法
对每只免疫介导型AA小鼠每天腹腔注射5毫克川芎嗪，共注射两次。在第14天，通过流式细胞术检测骨髓单个核细胞（MNC）中CD49d和CD49c的表达，通过免疫组织化学SABC法检测基质细胞上的血管细胞黏附分子-1（VCAM-1）。
结果
AA组中CD49d、CD49e和VCAM-1的表达明显低于正常组（t分别为11.96、17.18和7.09，P<0.001），且川芎嗪治疗组的表达明显高于未治疗组（t分别为9.05、14.61和6.91，P<0.001）。川芎嗪组中CD49d和VCAM-1的表达在正常范围内。
结论
川芎嗪可增加免疫介导型AA小鼠骨髓细胞黏附分子的表达，从而促进造血细胞的生长。
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