[草苁蓉甲醇提取物对二乙基亚硝胺诱导的大鼠肝前病变的抑制作用]
[Inhibitory effect of methanol extract of Boschniakia rossica Fedtsch. et Flerov on rat hepatic preneoplastic lesions induced by diethylnitrosamine].
作者信息
Yin Z Z, Jin H L, Li T Z, Lee J J, Kim Y H, Lee C H, Lee K H, Hyun B H
机构信息
Biochemistry Laboratory for Cancer Research, Yanbian University College of Medicine, Yanji 133000.
出版信息
Zhongguo Zhong Yao Za Zhi. 1998 Jul;23(7):424-6, 448- inside back cover.
OBJECTIVE
To investigate the inhibitory effect of Boschniakia rossica(BR) on hepatocarcinogenesis in rats.
METHOD
Based on immunohistochemistry techniques, the expression of placental form glutathione S-transferase(GST-P), mutant p53 and p21 protein were investigated in hepatic preneoplastic lesions induced by Solt-Farber protocol in the liver of rats that had been treated with the above method, administered with BR extract and of control group.
RESULT
The extract of BR(500 mg/kg) has inhibitory effect on the formation of diethylnitrosamine-induced GST-P-positive foci in F344 rat and the expression of mutant p53 and p21 protein was lower than that of hepatic preneoplastic lesions, and the increasing gamma-glutamyltranspeptidase(gamma-GT) activity in rat liver treated with Solt-Farber protocol was decreased by the extract of BR.
CONCLUSION
These results indicate that BR has inhibitory effect on DEN induced hepatic preneoplastic lesions in F344 rat.
目的
研究草苁蓉对大鼠肝癌发生的抑制作用。
方法
采用免疫组织化学技术，研究经上述方法处理、给予草苁蓉提取物的大鼠及对照组大鼠肝脏中经索尔特-法伯方案诱导的肝前病变中胎盘型谷胱甘肽S-转移酶（GST-P）、突变型p53和p21蛋白的表达。
结果
草苁蓉提取物（500mg/kg）对F344大鼠二乙基亚硝胺诱导的GST-P阳性灶的形成有抑制作用，突变型p53和p21蛋白的表达低于肝前病变，且草苁蓉提取物降低了经索尔特-法伯方案处理的大鼠肝脏中γ-谷氨酰转肽酶（γ-GT）活性的升高。
结论
这些结果表明草苁蓉对二乙基亚硝胺诱导的F344大鼠肝前病变有抑制作用。
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