Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, Punjab, India.
Nutr Cancer. 2011;63(2):234-41. doi: 10.1080/01635581.2011.523806.
Quercetin is an antioxidant flavonoid, found ubiquitously in nature and extensively used in herbal medicines and food additives. This study aimed to investigate the effect of quercetin on diethylnitrosamine-induced preneoplastic lesions, using the medium-term rat liver bioassay. The γ-benzene hexachloride was used as promoter at the doses of 0.1, 1.0, and 10.0 mg/kg against a single dose of diethylnitrosamine (200 mg/kg) in male Sprague-Dawley rats. All the rats were subjected to 70% partial hepatectomy at Week 4. The protective effect of quercetin (5 and 25 mg/kg) was examined against the highest dose of γ-benzene hexachloride (10 mg/kg). A significant increase in the number as well as the mean area of glutathione S-transferase placental form (GST-P) positive foci, p53 positive hepatocytes, and the percentage of apoptotic cells were observed in the diethylnitrosamine-treated group. In the present investigation, both doses of QC (5 and 25 mg/kg) led to a significant decrease in the number as well as the mean area of GST-P positive foci, TUNEL positive apoptotic cells, p53 positive hepatocytes, and restoration of cellular morphology. These results clearly indicate that quercetin inhibits diethylnitrosamine-induced hepatic preneoplastic lesions in medium-term rat liver bioassay.
槲皮素是一种抗氧化黄酮类化合物，广泛存在于自然界中，广泛用于草药和食品添加剂。本研究旨在通过中期大鼠肝生物测定法研究槲皮素对二乙基亚硝胺诱导的前肿瘤病变的影响。使用γ-苯六氯环己烷作为促进剂，剂量分别为 0.1、1.0 和 10.0mg/kg，对抗单次二乙基亚硝胺（200mg/kg）剂量，在雄性 Sprague-Dawley 大鼠中。所有大鼠在第 4 周进行 70%部分肝切除术。研究了槲皮素（5 和 25mg/kg）对最高剂量γ-苯六氯环己烷（10mg/kg）的保护作用。在二乙基亚硝胺处理组中，观察到谷胱甘肽 S-转移酶胎盘形式（GST-P）阳性焦点、p53 阳性肝细胞和凋亡细胞的百分比的数量以及平均面积显著增加。在本研究中，QC 的两个剂量（5 和 25mg/kg）均导致 GST-P 阳性焦点、TUNEL 阳性凋亡细胞、p53 阳性肝细胞的数量以及平均面积显著减少，并且细胞形态得到恢复。这些结果清楚地表明，槲皮素抑制中期大鼠肝生物测定中二乙基亚硝胺诱导的肝前肿瘤病变。
