[双苄基异喹啉Ys-96对人癌细胞阿霉素或长春新碱耐药性的体外逆转作用]
[Reversal effect of Ys-96, a bisbenzylisoquinoline, on adriamycin or vincristine resistance in human cancer cells in vitro].
作者信息
Ye Z, Sun A, Li C, Xue B, Wang Z, Tong Y, Feng H, Liang A, Wang J, Yang Q
机构信息
Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700.
出版信息
Zhongguo Zhong Yao Za Zhi. 1998 Jul;23(7):427-8, inside back cover.
OBJECTIVE
Reversal effect of Ys-96, a bisbenzylisoquinoline, on the resistance of human cancer cells to adriamycin or vincristine was studied in vitro.
METHOD
In an in vitro culture system of human cancer cells MCF-7 and its adriamycin-resistant line MCF-7/Ad or KB and its vincristine-resistant line KBv200, the sensitivity(IC50) of the resistant cell lines to adriamycin or vincristine was evaluated with a MTT assay.
RESULT
IC50 value of adriamycin or vincristine in combination with Ys-96 at a concentration of 1.00 mumol/L against MCF-7/Ad or KBv200 was found to be close to that of adriamycin alone or vincristine alone against the sensitive cell line MCF-7 or KB.
CONCLUSION
The drug resistance of MCF-7/Ad or KBv200 could be essentially reversed by Ys-96 at a concentration of 1.00 mumol/L.
目的
在体外研究双苄基异喹啉Ys - 96对人癌细胞对阿霉素或长春新碱耐药性的逆转作用。
方法
在人癌细胞MCF - 7及其阿霉素耐药株MCF - 7/Ad或KB及其长春新碱耐药株KBv200的体外培养系统中，采用MTT法评估耐药细胞株对阿霉素或长春新碱的敏感性（IC50）。
结果
发现阿霉素或长春新碱与浓度为1.00 μmol/L的Ys - 96联合使用时，对MCF - 7/Ad或KBv200的IC50值接近阿霉素单独或长春新碱单独对敏感细胞株MCF - 7或KB的IC50值。
结论
浓度为1.00 μmol/L的Ys - 96可基本逆转MCF - 7/Ad或KBv200的耐药性。
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