Kurokawa M, Ishida S, Asakawa S, Iwasa S, Goto N
Jpn J Med Sci Biol. 1975 Feb;28(1):37-52. doi: 10.7883/yoken1952.28.37.
Intraperitoneal or intravenous inoculation of live or inactivated influenza virus induced two characteristic responses of the peripheral leukocytes in mice, an early appearing leukopenic response and late appearing lymphopenia. The former response usually developed and subsided within several hours, though the change in leukocyte population was fairly complicated depending upon the activity of the inoculated material, while the latter began several hours after inoculation and reached its minimum level in 10 to 20 hr. The agent responsible for the former may be virus pyrogen, while the latter seems to be caused by some substance(s) other than that. The early appearing leukopenic response was similar to that due to bacterial endotoxin in respect to the characteristic pattern of the change in peripheral leukocyte population, though it was relatively easy to distinguish one from the other by the length of the latent period and by the heat stability of the causative agent. Live or inactivated influenza virus causing the early appearing leukopenic response was found also to have the mouse body weight-decreasing toxicity. The significance of these findings in the laboratory control test of influenza vaccine for untoward reactions often observed in human inoculated with some inactivated influenza vaccines was discussed. The possible roles of the two agents, virus pyrogen and endotoxin, in the febrile response were mentioned.
腹腔内或静脉内接种活的或灭活的流感病毒可诱导小鼠外周白细胞产生两种特征性反应，即早期出现的白细胞减少反应和晚期出现的淋巴细胞减少。前一种反应通常在数小时内出现并消退，尽管白细胞群体的变化相当复杂，这取决于接种物质的活性，而后一种反应在接种后数小时开始，并在10至20小时内达到最低水平。引起前一种反应的因素可能是病毒热原，而后一种反应似乎是由其他某种物质引起的。早期出现的白细胞减少反应在外周白细胞群体变化的特征模式方面与细菌内毒素引起的反应相似，尽管通过潜伏期的长短和病原体的热稳定性相对容易将两者区分开来。发现引起早期白细胞减少反应的活的或灭活的流感病毒也具有降低小鼠体重的毒性。讨论了这些发现在流感疫苗实验室对照试验中的意义，这些试验针对在接种某些灭活流感疫苗的人群中经常观察到的不良反应。提到了病毒热原和内毒素这两种因素在发热反应中的可能作用。
