Bozhenko L V, Agafonova L M, Konstantinova I I, Shefer L F, Kustikova Iu G
Vopr Virusol. 1983 Mar-Apr(2):187-91.
Biological properties of influenza vaccines were studied on experimental animals inoculated intraperitoneally with regard to the vaccine effect on peritoneal exudate cells. The development of neutrophilia and inhibition of acid phosphatase activity in macrophages within the first 24 hours after intraperitoneal inoculation of influenza viruses or influenza vaccines were used for determination of the toxic effect of the inoculum. The values of subsequent later activation of acid phosphatase in macrophages allowed the immunogenic properties of the vaccines under study to be evaluated. The sensitizing properties of the virus antigens were assessed by the reaction of the lymphoid-macrophage system after multiple inoculations of the preparations. A correlation of changes observed in the experimental animals with reactogenicity and values of immunity against influenza in man and animals was established.
在给实验动物腹腔接种流感疫苗后，就疫苗对腹腔渗出细胞的作用，对流感疫苗的生物学特性进行了研究。腹腔接种流感病毒或流感疫苗后24小时内，中性粒细胞增多以及巨噬细胞酸性磷酸酶活性受到抑制的情况，被用于确定接种物的毒性作用。巨噬细胞中酸性磷酸酶随后的激活值，有助于评估所研究疫苗的免疫原性。通过多次接种制剂后淋巴-巨噬细胞系统的反应，评估病毒抗原的致敏特性。确定了实验动物中观察到的变化与人和动物流感反应原性及免疫值之间的相关性。
