来自园芹的含聚腺苷酸（Poly(A)）RNA：体外分离、特性及信使功能

Poly(A)-containing RNA from Petroselinum hortense: isolation, properties and messenger function in vitro.

作者信息

Ragg H, Schroeder J, Hahlbrock K

出版信息

Mol Biol Rep. 1975 Jul;2(2):119-27. doi: 10.1007/BF00357542.

Abstract

Cell suspension cultures from parsley (Petroselinum hortense Hoffm.) were labelled in vivo with [2-3H] adenosine. The RNA isolated from the ribosomal pellet was fractionated on an oligo(dT)-cellulose column. Approximately 1.5% of the RNA, representing about 15% of the total radioactivity, was retained at high salt concentrations and eluted at low ionic strength. As determined by two independent methods, this fraction contained poly(A) segments with an average length of about 80 nucleotides. It was active as template in a cell-free system from wheat germ, directing the synthesis of peptides ranging in molecular weight from about 4000-40000 daltons.

摘要

用[2-³H]腺苷对欧芹（Petroselinum hortense Hoffm.）的细胞悬浮培养物进行体内标记。从核糖体沉淀中分离出的RNA在寡聚（dT）-纤维素柱上进行分级分离。约1.5%的RNA（占总放射性的约15%）在高盐浓度下保留，并在低离子强度下洗脱。通过两种独立方法测定，该级分含有平均长度约为80个核苷酸的聚（A）片段。它在小麦胚芽无细胞系统中作为模板具有活性，指导合成分子量约为4000 - 40000道尔顿的肽。