大豆短时间标记的单磷酸腺苷丰富的核糖核酸的特征。
Characterization of Short Time Labeled Adenosine Monophosphate-rich Ribonucleic Acids of Soybean.
机构信息
Department of Botany, University of Georgia, Athens, Georgia 30601.
出版信息
Plant Physiol. 1972 May;49(5):783-8. doi: 10.1104/pp.49.5.783.
Abstract
The total population of newly synthesized (32)P-AMP-rich RNA has been separated into two major types based on repeated fractionation on methylated albumin-kieselguhr columns. The purified D-RNA which elutes, under our experimental conditions, primarily in the salt gradient has a GMP/AMP ratio of about 0.8 and an AMP + UMP content of about 56 mole per cent. The purified TB-RNA which preferentially remains bound to the column in the salt gradient has a GMP/AMP ratio of about 0.4 to 0.45 and an AMP + UMP content of about 65 mole per cent. In addition to being distinguished by their fractionation on the methylated albumin-kieselguhr column and base composition analysis, purified D-RNA and TB-RNA have different size distributions on sucrose gradient and acrylamide gel fractionation, are differentially associated with polyribosomes and have different stabilities in the tissue.
摘要
已根据在甲硫氨酸白蛋白-硅藻土柱上的重复分级，将新合成的（32）P-AMP 丰富 RNA 的总群体分离成两种主要类型。在我们的实验条件下，主要在盐梯度中洗脱的纯化 D-RNA 的 GMP/AMP 比值约为 0.8，AMP+UMP 含量约为 56 摩尔%。优先保留在盐梯度中柱上的纯化 TB-RNA 的 GMP/AMP 比值约为 0.4 至 0.45，AMP+UMP 含量约为 65 摩尔%。除了在甲硫氨酸白蛋白-硅藻土柱上的分级和碱基组成分析方面有所区别外，纯化的 D-RNA 和 TB-RNA 在蔗糖梯度和丙烯酰胺凝胶分级方面的分布不同，与多核糖体的结合方式不同，在组织中的稳定性也不同。
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