Ganelina L Sh, Ovchinnikova L P, Ianushka A L
Tsitologiia. 1975 Jul;17(7):808-13.
The release and extraction of histones from the nuclei of rat liver under the influence of 0.005 M CaCl2 and 0.14 M NaCl was investigated. A considerable loss of nuclear histones, especially lysine-rich ones, was shown biochemically and cytophotometrically. The preliminary incubation of the nuclei in the respective salt solutions resulted in a more complete extraction of histones. This may be explained by a weaker binding capacity of histones to DNA under the latter conditions. Electrophoresis in polyacrylamid gel showed an increased mobility of histone fractions removed from the nuclei by incubation in the salt solutions.
研究了在0.005M氯化钙和0.14M氯化钠影响下，大鼠肝细胞核中组蛋白的释放和提取。生化和细胞光度法显示，核组蛋白有相当大的损失，尤其是富含赖氨酸的组蛋白。细胞核在相应盐溶液中的预孵育导致组蛋白的提取更完全。这可能是由于在后一种条件下组蛋白与DNA的结合能力较弱。聚丙烯酰胺凝胶电泳显示，通过在盐溶液中孵育从细胞核中去除的组蛋白组分迁移率增加。
