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狂犬病病毒的免疫化学特性:可沉淀抗原的测定

[Immunochemical characterization of rabies virus: determination of precipitable antigens].

作者信息

Zapata M T, Márquez A, Yantorno C, Riera M C, Scrimini C

出版信息

Rev Asoc Argent Microbiol. 1978 May-Aug;10(2):70-7.

PMID:116328
Abstract

Immunodiffusion and immuno-electrophoresis techniques were used to detect rabies precipitable antigens. The passive haemagglutination test with group "O" human red cells treated with tannic acid, was used as control test. The study was done with brain suspensions of rabies virus infected mice (CVS strain). Mouse and horse rabies antisera were used. The analysis of the results of both immunochemical assays showed the presence of two specific antigenic fractions of rabies virus. The same two fractions were detected when the antigens were heated. Experiments with brain extracts centrifuged at high speed, suggested that the antigenic fractions are of the "soluble" type.

摘要

采用免疫扩散和免疫电泳技术检测狂犬病可沉淀抗原。用经鞣酸处理的“O”型人红细胞进行被动血凝试验作为对照试验。该研究使用狂犬病病毒感染小鼠(CVS株)的脑悬液进行。使用了小鼠和马狂犬病抗血清。两种免疫化学分析结果显示狂犬病病毒存在两种特异性抗原成分。当抗原加热时也检测到相同的两种成分。对高速离心的脑提取物进行的实验表明,抗原成分属于“可溶性”类型。

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