Ferguson M, Seagroatt V, Schild G C
Dev Biol Stand. 1986;64:81-6.
The method currently recommended for the assay of rabies vaccine potency is a mouse protection test, the NIH test. It is widely accepted that the estimates of potency obtained in such tests are imprecise. We have applied the single radial immunodiffusion (SRD) technique to the assay of the glycoprotein antigen, the major protective antigen of the rabies virion. In this paper we have summarized the results of an international collaborative study, in which SRD was shown to give reproducible estimates of glycoprotein content. In addition the use of SRD in assessing the stability of rabies vaccines is discussed.
目前推荐用于检测狂犬病疫苗效力的方法是小鼠保护试验，即NIH试验。人们普遍认为，通过此类试验获得的效力估计值并不精确。我们已将单向辐射免疫扩散（SRD）技术应用于狂犬病病毒粒子主要保护性抗原糖蛋白抗原的检测。在本文中，我们总结了一项国际合作研究的结果，该研究表明SRD能够对糖蛋白含量给出可重复的估计值。此外，还讨论了SRD在评估狂犬病疫苗稳定性方面的应用。
