Araz M, Yilmaz N, Güngör K, Okan V, Kepekci Y, Sükrü Aynacioglu A
Department of Internal Medicine, Medical Faculty, Gaziantep University, TR-27070, Gaziantep, Turkey.
Diabetes Res Clin Pract. 2001 Nov;54(2):95-104. doi: 10.1016/s0168-8227(01)00257-1.
The aim of this study was to investigate whether an association exists between the angiotensin converting enzyme (ACE) insertion/deletion (I/D) polymorphism and microvascular complications of type 2 diabetes mellitus in Turkish patients. A total of 239 type 2 diabetic patients and 138 sex and age matched control subjects were included into the study. The I/D polymorphism was determined by polymerase chain reaction (PCR). Nephropathy status was determined according to urinary albumin/creatinine ratio (microg/mg) (<30 normoalbuminuria, 30-300 microalbuminuria, >300 macroalbuminuria) and retinopathy was evaluated by fundoscopic examination and by flourescein fundus angiography. The distribution of ACE I/D polymorphism and allele frequencies in diabetic patients were not significantly different from controls, DD genotype 32.2 versus 37.2%; ID genotype 50.6 versus 47.1%; and II 17.2 versus 15.2%; D allele 57.5 versus 61.2%; I allele 42.5 versus 38.8%. Genotype distribution between normo-, micro- and macroalbuminuric patients did not differ significantly (DD:ID:II (%), normoalbuminuria, 35:46:19; microalbuminuria, 28:55:17; macroalbuminuria, 31:55:14). There was also no difference in genotype distribution between patients with and without retinopathy (DD:ID:II (%), retinopathy positive, 32:51:17; retinopathy negative, 33:49:18). In conclusion, the ACE I/D polymorphism does not seem to be associated with diabetic nephropathy and retinopathy in Turkish type 2 diabetic patients.
本研究旨在调查土耳其患者中血管紧张素转换酶（ACE）插入/缺失（I/D）多态性与2型糖尿病微血管并发症之间是否存在关联。共有239例2型糖尿病患者和138例年龄及性别匹配的对照者纳入本研究。通过聚合酶链反应（PCR）确定I/D多态性。根据尿白蛋白/肌酐比值（微克/毫克）（<30为正常白蛋白尿，30 - 300为微量白蛋白尿，>300为大量白蛋白尿）确定肾病状态，通过眼底镜检查和荧光素眼底血管造影评估视网膜病变。糖尿病患者中ACE I/D多态性和等位基因频率的分布与对照组无显著差异，DD基因型分别为32.2%对37.2%；ID基因型分别为50.6%对47.1%；II基因型分别为17.2%对15.2%；D等位基因分别为57.5%对61.2%；I等位基因分别为42.5%对38.8%。正常白蛋白尿、微量白蛋白尿和大量白蛋白尿患者之间的基因型分布无显著差异（DD:ID:II（%），正常白蛋白尿组为35:46:19；微量白蛋白尿组为28:55:17；大量白蛋白尿组为31:55:14）。有视网膜病变和无视网膜病变患者之间的基因型分布也无差异（DD:ID:II（%），视网膜病变阳性组为32:51:17；视网膜病变阴性组为33:49:18）。总之，在土耳其2型糖尿病患者中，ACE I/D多态性似乎与糖尿病肾病和视网膜病变无关。
