Kochieva E Z, Goriunova S V, Pomortsev A A
Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991 Russia.
Genetika. 2001 Aug;37(8):1088-94.
Random amplification of polymorphic DNA (RAPD) was used to analyze six species, three populations, and seven regional cultivars of barley. A unique pattern of amplified DNA products was obtained for each species of the genus Hordeum. High polymorphism of barley species was revealed. Specific fragments were found in most RAPD patterns; the fragments can be used as molecular markers of corresponding species and subspecies. Several other DNA fragments were shown to serve as molecular markers of the H genome. Specific RAPD patterns were obtained for each population and each cultivar of H. vulgare sensu lato. In total, variation between the populations and between the cultivars was substantially lower than between species. Cluster analysis (UPGMA) was used to estimate genetic distances between the Hordeum species, between the H. spontaneum populations, and between regional H. vulgare cultivars and a dendrogram was constructed.
采用随机扩增多态性DNA（RAPD）技术对大麦的6个物种、3个居群和7个地方品种进行了分析。每个大麦属物种都获得了独特的DNA扩增产物图谱。揭示了大麦物种的高度多态性。在大多数RAPD图谱中发现了特异性片段；这些片段可作为相应物种和亚种的分子标记。还发现其他几个DNA片段可作为H基因组的分子标记。对宽颖大麦的每个居群和每个品种都获得了特异性RAPD图谱。总体而言，居群间和品种间的变异明显低于物种间的变异。采用聚类分析（UPGMA）估算大麦物种间、野生大麦居群间以及地方栽培大麦品种间的遗传距离，并构建了聚类图。
