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不同物种的牛奶和血清中的脱氧核糖核酸酶。

DNases in milk and blood sera from different species.

作者信息

Gudding R

出版信息

Acta Vet Scand. 1979;20(3):404-16. doi: 10.1186/BF03546602.

DOI:10.1186/BF03546602
PMID:116487
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8322905/
Abstract

DNases were demonstrated in samples of colostrum and blood serum from man and various domestic animals. The measurable DNase activity recorded was highest in samples from cat and dog and lowest in samples from goat, horse, pig and sheep. In contrast to DNases produced by certain bacteria, these enzymes were thermo-labile and the activity was maximal in the area pH 5.0–5.5. A modification of an agar medium originally described for the demonstration of bacterial DNases was found to be suitable for assays of DNases from colostrum, milk and serum.

摘要

在人和各种家畜的初乳和血清样本中均检测到了脱氧核糖核酸酶(DNases)。所记录的可测量的DNase活性在猫和狗的样本中最高,而在山羊、马、猪和绵羊的样本中最低。与某些细菌产生的DNases不同,这些酶对热不稳定,且在pH 5.0 - 5.5范围内活性最大。人们发现,一种最初用于检测细菌DNases的琼脂培养基经过改良后,适用于检测初乳、牛奶和血清中的DNases。

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本文引用的文献

1
STUDIES ON ACID DEOXYRIBONUCLEASE. II. ISOLATION AND CHARACTERIZATION OF SPLEEN-ACID DEOXYRIBONUCLEASE.酸性脱氧核糖核酸酶的研究。II. 脾脏酸性脱氧核糖核酸酶的分离与特性
Biochemistry. 1964 Oct;3:1419-26. doi: 10.1021/bi00898a005.
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Acid deoxyribonuclease activity in purified calf thymus nuclei.纯化小牛胸腺细胞核中的酸性脱氧核糖核酸酶活性
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Serum deoxyribonuclease I and its inhibitor in growing and pregnant rats.生长中和妊娠大鼠血清中的脱氧核糖核酸酶I及其抑制剂
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A comparative study of acid deoxyribonucleases extracted from different tissues and species.从不同组织和物种中提取的酸性脱氧核糖核酸酶的比较研究。
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Quantitative determination of proteolytic, lipolytic and haemolytic activities in semen of some animal species.某些动物物种精液中蛋白水解、脂肪水解和溶血活性的定量测定。
Acta Vet Scand. 1965;6(4):318-28. doi: 10.1186/BF03547089.
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Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity.用于检测葡萄球菌核酸酶活性的异染琼脂扩散法。
Appl Microbiol. 1971 Apr;21(4):585-7. doi: 10.1128/am.21.4.585-587.1971.
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A protein inhibitor of acid deoxyribonucleases.酸性脱氧核糖核酸酶的一种蛋白质抑制剂。
Proc Natl Acad Sci U S A. 1969 Nov;64(3):913-9. doi: 10.1073/pnas.64.3.913.
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Anal Biochem. 1972 Apr;46(2):502-33. doi: 10.1016/0003-2697(72)90324-7.
10
Studies on acid hydrolases. I. A procedure for the preparation of acid deoxyribonuclease and other acid hydrolases.酸性水解酶的研究。I. 一种制备酸性脱氧核糖核酸酶及其他酸性水解酶的方法。
Biochim Biophys Acta. 1966 Oct 24;129(1):1-11.