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不同物种的牛奶和血清中的脱氧核糖核酸酶。

DNases in milk and blood sera from different species.

作者信息

Gudding R

出版信息

Acta Vet Scand. 1979;20(3):404-16. doi: 10.1186/BF03546602.

Abstract

DNases were demonstrated in samples of colostrum and blood serum from man and various domestic animals. The measurable DNase activity recorded was highest in samples from cat and dog and lowest in samples from goat, horse, pig and sheep. In contrast to DNases produced by certain bacteria, these enzymes were thermo-labile and the activity was maximal in the area pH 5.0–5.5. A modification of an agar medium originally described for the demonstration of bacterial DNases was found to be suitable for assays of DNases from colostrum, milk and serum.

摘要

在人和各种家畜的初乳和血清样本中均检测到了脱氧核糖核酸酶(DNases)。所记录的可测量的DNase活性在猫和狗的样本中最高,而在山羊、马、猪和绵羊的样本中最低。与某些细菌产生的DNases不同,这些酶对热不稳定,且在pH 5.0 - 5.5范围内活性最大。人们发现,一种最初用于检测细菌DNases的琼脂培养基经过改良后,适用于检测初乳、牛奶和血清中的DNases。

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本文引用的文献

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A protein inhibitor of acid deoxyribonucleases.酸性脱氧核糖核酸酶的一种蛋白质抑制剂。
Proc Natl Acad Sci U S A. 1969 Nov;64(3):913-9. doi: 10.1073/pnas.64.3.913.
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Production and heat stability of staphylococcal nuclease.葡萄球菌核酸酶的产生及热稳定性
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