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基于核酸酶特性对葡萄球菌进行鉴别。

Differentiation of staphylococci on the basis of nuclease properties.

作者信息

Gudding R

出版信息

J Clin Microbiol. 1983 Nov;18(5):1098-101. doi: 10.1128/jcm.18.5.1098-1101.1983.

DOI:10.1128/jcm.18.5.1098-1101.1983
PMID:6643663
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC272848/
Abstract

The quantity, thermostability, and serological pattern of nucleases produced by different staphylococci were studied. Staphylococcal strains were isolated from nine different species of animals or from humans. Staphylococcus aureus, Staphylococcus intermedius, and Staphylococcus hyicus subsp. hyicus were vigorous producers of nuclease, whereas the coagulase-negative staphylococci, except S. hyicus subsp. hyicus, produced significantly less nuclease. The nucleases of all strains were found to be thermostable. S. aureus, S. intermedius, and S. hyicus subsp. hyicus could be distinguished from each other and from coagulase-negative staphylococci on the basis of inhibition of nuclease activity by specific antibodies.

摘要

研究了不同葡萄球菌产生的核酸酶的数量、热稳定性和血清学模式。从九种不同动物或人类中分离出葡萄球菌菌株。金黄色葡萄球菌、中间葡萄球菌和猪葡萄球菌猪亚种是核酸酶的大量生产者,而除猪葡萄球菌猪亚种外的凝固酶阴性葡萄球菌产生的核酸酶明显较少。发现所有菌株的核酸酶都具有热稳定性。基于特异性抗体对核酸酶活性的抑制作用,金黄色葡萄球菌、中间葡萄球菌和猪葡萄球菌猪亚种可以相互区分,也可以与凝固酶阴性葡萄球菌区分开来。

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Differentiation of staphylococci on the basis of nuclease properties.基于核酸酶特性对葡萄球菌进行鉴别。
J Clin Microbiol. 1983 Nov;18(5):1098-101. doi: 10.1128/jcm.18.5.1098-1101.1983.
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本文引用的文献

1
Identification of coagulase-positive Staphylococcus intermedius and Staphylococcus hyicus subsp. hyicus isolates from veterinary clinical specimens.从兽医临床样本中鉴定凝固酶阳性中间葡萄球菌和猪葡萄球菌猪葡萄球菌亚种。
J Clin Microbiol. 1981 Dec;14(6):671-3. doi: 10.1128/jcm.14.6.671-673.1981.
2
Nuclease of staphylococcus epidermidis isolated from mastitic milk. Production and some properties.从患乳腺炎奶牛的乳汁中分离出的表皮葡萄球菌核酸酶。生产及某些特性。
Acta Vet Scand. 1980;21(2):267-77. doi: 10.1186/BF03546889.
3
Identification of pathogenic staphylococci isolated from animals and foods derived from animals.从动物及动物源性食品中分离出的致病性葡萄球菌的鉴定。
J Appl Bacteriol. 1980 Aug;49(1):1-11. doi: 10.1111/j.1365-2672.1980.tb01038.x.
4
Identification of Staphylococcus species with the API STAPH-IDENT system.使用API STAPH-IDENT系统鉴定葡萄球菌属菌种。
J Clin Microbiol. 1982 Sep;16(3):509-16. doi: 10.1128/jcm.16.3.509-516.1982.
5
Differentiation of strains of Staphylococcus epidermidis isolated from bovine udders.从牛乳房分离出的表皮葡萄球菌菌株的鉴别
J Gen Microbiol. 1967 May;47(2):273-87. doi: 10.1099/00221287-47-2-273.
6
Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity.用于检测葡萄球菌核酸酶活性的异染琼脂扩散法。
Appl Microbiol. 1971 Apr;21(4):585-7. doi: 10.1128/am.21.4.585-587.1971.
7
Production and heat stability of staphylococcal nuclease.葡萄球菌核酸酶的产生及热稳定性
Appl Microbiol. 1973 Mar;25(3):332-6. doi: 10.1128/am.25.3.332-336.1973.
8
Differentiation of staphylococcal and micrococcal proteinases by electrophoresis.通过电泳鉴别葡萄球菌蛋白酶和微球菌蛋白酶。
Appl Microbiol. 1974 Nov;28(5):768-73. doi: 10.1128/am.28.5.768-773.1974.
9
The occurrence of antibodies against staphylococcal deoxyribonucleases in blood sera from different species.不同物种血清中抗葡萄球菌脱氧核糖核酸酶抗体的出现情况。
Acta Vet Scand. 1974;15(4):631-5. doi: 10.1186/BF03547232.
10
Simplified scheme for routine identification of human Staphylococcus species.人类葡萄球菌属常规鉴定的简化方案。
J Clin Microbiol. 1975 Jan;1(1):82-8. doi: 10.1128/jcm.1.1.82-88.1975.