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基于核酸酶特性对葡萄球菌进行鉴别。

Differentiation of staphylococci on the basis of nuclease properties.

作者信息

Gudding R

出版信息

J Clin Microbiol. 1983 Nov;18(5):1098-101. doi: 10.1128/jcm.18.5.1098-1101.1983.

Abstract

The quantity, thermostability, and serological pattern of nucleases produced by different staphylococci were studied. Staphylococcal strains were isolated from nine different species of animals or from humans. Staphylococcus aureus, Staphylococcus intermedius, and Staphylococcus hyicus subsp. hyicus were vigorous producers of nuclease, whereas the coagulase-negative staphylococci, except S. hyicus subsp. hyicus, produced significantly less nuclease. The nucleases of all strains were found to be thermostable. S. aureus, S. intermedius, and S. hyicus subsp. hyicus could be distinguished from each other and from coagulase-negative staphylococci on the basis of inhibition of nuclease activity by specific antibodies.

摘要

研究了不同葡萄球菌产生的核酸酶的数量、热稳定性和血清学模式。从九种不同动物或人类中分离出葡萄球菌菌株。金黄色葡萄球菌、中间葡萄球菌和猪葡萄球菌猪亚种是核酸酶的大量生产者,而除猪葡萄球菌猪亚种外的凝固酶阴性葡萄球菌产生的核酸酶明显较少。发现所有菌株的核酸酶都具有热稳定性。基于特异性抗体对核酸酶活性的抑制作用,金黄色葡萄球菌、中间葡萄球菌和猪葡萄球菌猪亚种可以相互区分,也可以与凝固酶阴性葡萄球菌区分开来。

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