一种用于测定抗金黄色葡萄球菌脱氧核糖核酸酶抗体的琼脂扩散法。
An agar diffusion method for the determination of antibodies against Staphylococcus aureus deoxyribonuclease.
作者信息
Gudding R
出版信息
Acta Vet Scand. 1977;18(4):480-93. doi: 10.1186/BF03548411.
Abstract
On the addition of small concentrations of deoxyribonuclease, produced by Staphylococcus aureus, to Toluidine Blue DNA agar, a medium is produced on which antibodies against S. aureus deoxyribonuclease may be detected. When samples of milk, or blood serum, containing antibodies against S. aureus are applied into wells in the agar, the deoxyribonuclease activity is inhibited by the antibodies diffusing into the agar. As a result of this inhibition, blue zones are produced around the wells in the otherwise bluish-red agar. The diameters of the zones correspond to the concentrations of antibodies, and the method may consequently be used for qualitative and quantitative examinations of antibodies against S. aureus deoxyribonuclease in milk and serum. The procedure and certain limitations of the method are described.
摘要
向甲苯胺蓝DNA琼脂中添加少量由金黄色葡萄球菌产生的脱氧核糖核酸酶，会制成一种培养基，在该培养基上可检测到针对金黄色葡萄球菌脱氧核糖核酸酶的抗体。当将含有针对金黄色葡萄球菌抗体的牛奶或血清样本加入琼脂中的孔中时，扩散到琼脂中的抗体可抑制脱氧核糖核酸酶的活性。由于这种抑制作用，在原本呈蓝红色的琼脂中孔周围会产生蓝色区域。这些区域的直径与抗体浓度相对应，因此该方法可用于定性和定量检测牛奶和血清中针对金黄色葡萄球菌脱氧核糖核酸酶的抗体。本文描述了该方法的步骤及某些局限性。
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Proteolytic enzymes and biological inhibitors. I. Comparison between the Kunitz method and the agar gel casein precipitating reaction for determination of the activity of some commercial proteolytic enzymes and inhibitors.蛋白水解酶与生物抑制剂。I. 用于测定某些商业蛋白水解酶和抑制剂活性的库尼茨方法与琼脂凝胶酪蛋白沉淀反应之间的比较。
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