Jonsson H T, Culp T W, Kaufman R H, Smythe A, Feldman G L
Proc Soc Exp Biol Med. 1975 Sep;149(4):1005-9. doi: 10.3181/00379727-149-38944.
The influence of exogenous PMS and/or HCG, on the arachidonic acid (C 20:4omega6) content of the immature rat ovary was examined. Changes in ovarian arachidonate content associated with hormone administration were assessed in total lipid extracts, and in several neutral and phospholipid fractions. Both relative percentage and absolute amounts of arachidonic acid in several lipids were measured as well as uptake of radioactivity into total lipid resulting from the administration of 3H-labeled arachidonic acid in vivo. On the basis of these studies, we conclude (1) PMS, with or without HCG promotes increased uptake of exogenous arachidonic acid into ovarian total lipids; (2) Arachidonic acid is a mojor fatty acid constituent from noncholine containing phosphatides at the onset of normal estrous (ca. 38 days) even in the animals which received no PMS or HCG; (3) Changes in ovarian arachidonic acid levels following gonadotropin administration are more striking in the two phospholipid fractions than in the two neutral lips examined; (4) PMS is associated with a rapid outpouring of ovarian lipid, accompanied by a high turnover of arachidonic acid which is enhanced or modified temporally by added HCG in vivo. These results provide the first quantitative evidence that gonadotropins may regulate prostaglandin biosynthesis in the ovary by their effects on the uptake, storage, or release of arachidonic acid, a major PG precursor, from specific ovarian lipids. While the data strongly suggest that the regulation of one or more ovarian esterases (cholesterol esterase, lipase, phospholipase) is the mechanism by which gonadotropins regulate PG biosynthesis, a direct action on PG synthetase is not ruled out.
研究了外源性孕马血清促性腺激素（PMS）和/或人绒毛膜促性腺激素（HCG）对未成熟大鼠卵巢花生四烯酸（C20:4ω6）含量的影响。在总脂质提取物以及几种中性和磷脂组分中评估了与激素给药相关的卵巢花生四烯酸含量变化。测定了几种脂质中花生四烯酸的相对百分比和绝对含量，以及体内给予3H标记花生四烯酸后总脂质中放射性的摄取情况。基于这些研究，我们得出以下结论：（1）无论有无HCG，PMS均可促进外源性花生四烯酸摄取进入卵巢总脂质；（2）即使在未接受PMS或HCG的动物中，花生四烯酸也是正常发情初期（约38天）含非胆碱磷脂的主要脂肪酸成分；（3）促性腺激素给药后，卵巢花生四烯酸水平的变化在两种磷脂组分中比在所检测的两种中性脂质中更为显著；（4）PMS与卵巢脂质的快速释放有关，伴随着花生四烯酸的高周转率，体内添加的HCG可在时间上增强或改变这种周转率。这些结果提供了首个定量证据，表明促性腺激素可能通过影响花生四烯酸（一种主要的前列腺素前体）从特定卵巢脂质中的摄取、储存或释放来调节卵巢中前列腺素的生物合成。虽然数据强烈表明一种或多种卵巢酯酶（胆固醇酯酶、脂肪酶、磷脂酶）的调节是促性腺激素调节前列腺素生物合成的机制，但不排除对前列腺素合成酶的直接作用。
