Strother W N, Chernet E J, Lumeng L, Li T K, McBride W J
Department of Psychiatry, Institute of Psychiatric Research, 791 Union Drive, Indianapolis, IN 46202-4887, USA.
Alcohol. 2001 Aug;25(1):31-8. doi: 10.1016/s0741-8329(01)00162-8.
The densities of delta-opioid receptors in the central nervous system of alcohol-naive, adult, male, alcohol-preferring P, alcohol-nonpreferring NP, and Wistar rats were examined with the use of quantitative autoradiography. Slides with coronal 20-microm sections through the regions of interest were incubated in 5 nM [3H]-[D-Pen(2),D-Pen(5)]enkephalin (DPDPE) to label delta(1)-opioid receptor sites. Nonspecific binding was determined in the presence of 10 microM naloxone. Significant differences between the P and the NP rat lines were found in numerous cortical regions, the basolateral amygdala, and the posterior hippocampus, with 10%-20% lower [3H]-DPDPE binding found in the P line. In most regions examined, binding levels in the Wistar rats were intermediate between those of the P and the NP rats. Significantly lower [3H]-DPDPE binding levels in the P rat may indicate fewer delta(1)-opioid receptors or decreased binding affinity. The lower binding in certain limbic regions, such as the basolateral amygdala and posterior hippocampus, as well as cortical differences in the P rat may be associated with the divergent alcohol drinking behaviors found between the P and the NP lines.
利用定量放射自显影技术，检测了未接触过酒精的成年雄性嗜酒P大鼠、非嗜酒NP大鼠和Wistar大鼠中枢神经系统中δ-阿片受体的密度。将含有感兴趣区域冠状20微米切片的载玻片在5 nM [3H]-[D-青霉胺(2)，D-青霉胺(5)]脑啡肽(DPDPE)中孵育，以标记δ(1)-阿片受体位点。在10 μM纳洛酮存在的情况下测定非特异性结合。在许多皮质区域、基底外侧杏仁核和后海马体中发现P大鼠品系和NP大鼠品系之间存在显著差异，P大鼠品系中[3H]-DPDPE结合降低了10%-20%。在大多数检测区域中，Wistar大鼠的结合水平介于P大鼠和NP大鼠之间。P大鼠中[3H]-DPDPE结合水平显著降低可能表明δ(1)-阿片受体数量减少或结合亲和力降低。P大鼠某些边缘区域（如基底外侧杏仁核和后海马体）的结合较低以及皮质差异可能与P大鼠品系和NP大鼠品系之间不同的饮酒行为有关。
