可逆聚合溶质凝胶色谱中浓度依赖性迁移的定量解释。
Quantitative interpretation of concentration-dependent migration in gel chromatography of reversibly polymerizing solutes.
作者信息
Baghurst P A, Nichol L W, Ogston A G, Winzor D J
出版信息
Biochem J. 1975 Jun;147(3):575-83. doi: 10.1042/bj1470575.
Abstract
A theoretical expression is derived for concentration dependence of elution volume in the gel chromatography of a non-interacting solute. Experimental results for bovine serum albumin on Sephadex G-100 are shown to be in good agreement with the predicted gel-chromatographic behaviour. The theoretical treatment of concentration dependence is extended to include a solute undergoing rapid reversible polymerization (nA in equilibrium C). Computer simulation of gel-chromatographic data for monomer-dimer systems on Sephadex G-100 is used to illustrate the deficiencies of earlier empirical approaches, and also the potential of the present treatment, of allowing for non-chemical concentration dependence in gel chromatography of polymerizing solutes.
摘要
推导出了非相互作用溶质在凝胶色谱中洗脱体积对浓度依赖性的理论表达式。牛血清白蛋白在葡聚糖凝胶G - 100上的实验结果表明与预测的凝胶色谱行为高度吻合。浓度依赖性的理论处理扩展到包括经历快速可逆聚合的溶质（nA处于平衡C）。对葡聚糖凝胶G - 100上单体 - 二聚体系统的凝胶色谱数据进行计算机模拟，以说明早期经验方法的不足，以及本处理方法在考虑聚合溶质凝胶色谱中非化学浓度依赖性方面的潜力。
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