哺乳动物细胞亚染色体单位上DNA复制的调控
Regulation of DNA replication on subchromosomal units of mammalian cells.
作者信息
Hand R
出版信息
J Cell Biol. 1975 Jan;64(1):89-97. doi: 10.1083/jcb.64.1.89.
Abstract
The regulation of DNA replication at a subchromosomal level in mammalian cells has been investigated. DNA fiber autoradiographs were prepared from mouse L-929 cells pulse labeled with (3H)thymidine. Initiation events and subsequent chain growth occurring over short stretches (up to three replication units in length) of chromosomal DNA were analyzed. The results show that adjacent units usually initiate replication synchronously and that this synchrony is related to the proximity of initiation sites. In addition, adjacent units are of similar size and the rates of replication fork progression within units and on adjacent units are similar. The rate of fork progression increases with increasing replication unit size. Finally, no evidence for fixed termination sites for the units has been found. These observations suggest that despite large variations in size of replication units, timing of initiation events, and rates of fork progression found in chromosomal DNA as a whole, these processes are closely regulated within subchromosomal clusters of active replication units.
摘要
对哺乳动物细胞亚染色体水平上DNA复制的调控进行了研究。用（3H）胸腺嘧啶脉冲标记小鼠L-929细胞，制备DNA纤维放射自显影片。分析了染色体DNA短片段（长度达三个复制单元）上发生的起始事件和随后的链生长。结果表明，相邻单元通常同步起始复制，且这种同步性与起始位点的接近程度有关。此外，相邻单元大小相似，单元内和相邻单元上复制叉前进的速率相似。复制叉前进的速率随复制单元大小的增加而增加。最后，未发现单元有固定终止位点的证据。这些观察结果表明，尽管在整个染色体DNA中复制单元大小、起始事件时间和复制叉前进速率存在很大差异，但在活跃复制单元的亚染色体簇内，这些过程受到密切调控。
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