tagD的精确缺失及其产物甘油3-磷酸胞苷转移酶的可控消耗，会导致枯草芽孢杆菌在生理温度下生长时形态不规则并裂解。
Precise deletion of tagD and controlled depletion of its product, glycerol 3-phosphate cytidylyltransferase, leads to irregular morphology and lysis of Bacillus subtilis grown at physiological temperature.
作者信息
Bhavsar A P, Beveridge T J, Brown E D
机构信息
Antimicrobial Research Centre, Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada L8N 3Z5.
出版信息
J Bacteriol. 2001 Nov;183(22):6688-93. doi: 10.1128/JB.183.22.6688-6693.2001.
Abstract
Using a previously reported conditional expression system for use in Bacillus subtilis (A. P. Bhavsar, X. Zhao, and E. D. Brown, Appl. Environ. Microbiol. 67:403-410, 2001), we report the first precise deletion of a teichoic acid biosynthesis (tag) gene, tagD, in B. subtilis. This teichoic acid mutant showed a lethal phenotype when characterized at a physiological temperature and in a defined genetic background. This tagD mutant was subject to full phenotypic rescue upon expression of the complementing copy of tagD. Depletion of the tagD gene product (glycerol 3-phosphate cytidylyltransferase) via modulated expression of tagD from the amyE locus revealed structural defects centered on shape, septation, and division. Thickening of the wall and ultimately lysis followed these events.
摘要
利用先前报道的用于枯草芽孢杆菌的条件表达系统（A. P. 巴夫萨尔、X. 赵和E. D. 布朗，《应用与环境微生物学》67:403 - 410，2001年），我们报道了枯草芽孢杆菌中首个精确缺失磷壁酸生物合成（tag）基因tagD的情况。这个磷壁酸突变体在生理温度和特定遗传背景下进行表征时表现出致死表型。当表达tagD的互补拷贝时，这个tagD突变体得到了完全的表型拯救。通过从amyE位点调节tagD的表达来消耗tagD基因产物（甘油3 - 磷酸胞苷转移酶），揭示了以形状、隔膜形成和分裂为中心的结构缺陷。这些事件之后是细胞壁增厚并最终裂解。
相似文献
1
Precise deletion of tagD and controlled depletion of its product, glycerol 3-phosphate cytidylyltransferase, leads to irregular morphology and lysis of Bacillus subtilis grown at physiological temperature.tagD的精确缺失及其产物甘油3-磷酸胞苷转移酶的可控消耗，会导致枯草芽孢杆菌在生理温度下生长时形态不规则并裂解。
J Bacteriol. 2001 Nov;183(22):6688-93. doi: 10.1128/JB.183.22.6688-6693.2001.
2
Development and characterization of a xylose-dependent system for expression of cloned genes in Bacillus subtilis: conditional complementation of a teichoic acid mutant.枯草芽孢杆菌中用于克隆基因表达的木糖依赖性系统的开发与特性研究：磷壁酸突变体的条件互补
Appl Environ Microbiol. 2001 Jan;67(1):403-10. doi: 10.1128/AEM.67.1.403-410.2001.
3
Teichoic acid is an essential polymer in Bacillus subtilis that is functionally distinct from teichuronic acid.磷壁酸是枯草芽孢杆菌中的一种必需聚合物，其功能与磷壁醛酸不同。
J Bacteriol. 2004 Dec;186(23):7865-73. doi: 10.1128/JB.186.23.7865-7873.2004.
4
A conditional-lethal mutant of bacillus subtilis 168 with a thermosensitive glycerol-3-phosphate cytidylyltransferase, an enzyme specific for the synthesis of the major wall teichoic acid.枯草芽孢杆菌168的一种条件致死突变体，其具有对温度敏感的甘油-3-磷酸胞苷酰转移酶，该酶是合成主要壁磷壁酸所特有的一种酶。
J Gen Microbiol. 1991 Apr;137(4):921-8. doi: 10.1099/00221287-137-4-921.
5
CTP:glycerol 3-phosphate cytidylyltransferase (TarD) from Staphylococcus aureus catalyzes the cytidylyl transfer via an ordered Bi-Bi reaction mechanism with micromolar K(m) values.来自金黄色葡萄球菌的CTP：甘油3-磷酸胞苷转移酶（TarD）通过有序的双底物双产物反应机制催化胞苷转移，其米氏常数（K(m)）值为微摩尔级别。
Biochim Biophys Acta. 2003 Mar 21;1646(1-2):196-206. doi: 10.1016/s1570-9639(03)00019-0.
6
The Amino terminus of Bacillus subtilis TagB possesses separable localization and functional properties.枯草芽孢杆菌TagB的氨基末端具有可分离的定位和功能特性。
J Bacteriol. 2007 Oct;189(19):6816-23. doi: 10.1128/JB.00910-07. Epub 2007 Jul 27.
7
Wall teichoic acid polymers are dispensable for cell viability in Bacillus subtilis.壁磷壁酸聚合物对于枯草芽孢杆菌的细胞活力而言并非必需。
J Bacteriol. 2006 Dec;188(23):8313-6. doi: 10.1128/JB.01336-06. Epub 2006 Sep 29.
8
Crystal structure of CTP:glycerol-3-phosphate cytidylyltransferase from Staphylococcus aureus: examination of structural basis for kinetic mechanism.金黄色葡萄球菌CTP：甘油-3-磷酸胞苷酰转移酶的晶体结构：动力学机制的结构基础研究
Biochim Biophys Acta. 2006 Jan;1764(1):63-9. doi: 10.1016/j.bbapap.2005.10.015. Epub 2005 Nov 10.
9
In Bacillus subtilis 168, teichoic acid of the cross-wall may be different from that of the cylinder: a hypothesis based on transcription analysis of tag genes.在枯草芽孢杆菌168中，横壁的磷壁酸可能与柱状部分的磷壁酸不同：基于tag基因转录分析的假说。
Microbiology (Reading). 1995 Oct;141 ( Pt 10):2379-89. doi: 10.1099/13500872-141-10-2379.
10
Autoregulation of subtilin biosynthesis in Bacillus subtilis: the role of the spa-box in subtilin-responsive promoters.枯草芽孢杆菌中枯草菌素生物合成的自动调节：spa-box在枯草菌素反应性启动子中的作用。
Peptides. 2004 Sep;25(9):1415-24. doi: 10.1016/j.peptides.2003.11.025.
引用本文的文献
1
Mirubactin C rescues the lethal effect of cell wall biosynthesis mutations in .微小菌素C挽救了[具体生物]中细胞壁生物合成突变的致死效应。
Front Microbiol. 2022 Oct 13;13:1004737. doi: 10.3389/fmicb.2022.1004737. eCollection 2022.
2
A genetic screen to identify factors affected by undecaprenyl phosphate recycling uncovers novel connections to morphogenesis in Escherichia coli.一个遗传筛选实验，旨在识别受十一烯基磷酸循环影响的因素，揭示了大肠杆菌形态发生过程中的新的关联。
Mol Microbiol. 2021 Feb;115(2):191-207. doi: 10.1111/mmi.14609. Epub 2020 Oct 12.
3
Polysaccharide length affects mycobacterial cell shape and antibiotic susceptibility.多糖长度影响分枝杆菌细胞形态和抗生素敏感性。
Sci Adv. 2020 Sep 16;6(38). doi: 10.1126/sciadv.aba4015. Print 2020 Sep.
4
Galactosylated wall teichoic acid, but not lipoteichoic acid, retains InlB on the surface of serovar 4b Listeria monocytogenes.半乳糖基化细胞壁磷壁酸，但不是脂磷壁酸，可保留血清型 4b 李斯特菌表面的 InlB。
Mol Microbiol. 2020 Mar;113(3):638-649. doi: 10.1111/mmi.14455.
5
Disruption of l-Rhamnose Biosynthesis Results in Severe Growth Defects in Streptococcus mutans.l-鼠李糖生物合成的破坏导致变形链球菌的严重生长缺陷。
J Bacteriol. 2020 Feb 25;202(6). doi: 10.1128/JB.00728-19.
6
Periplasmic protein EipA determines envelope stress resistance and virulence in Brucella abortus.周质蛋白 EipA 决定布鲁氏菌属中布鲁氏菌的包膜应激抗性和毒力。
Mol Microbiol. 2019 Mar;111(3):637-661. doi: 10.1111/mmi.14178. Epub 2019 Jan 21.
7
Comparative genomic analysis of the flagellin glycosylation island of the Gram-positive thermophile Geobacillus.革兰氏阳性嗜热栖热放线菌鞭毛蛋白糖基化岛的比较基因组分析
BMC Genomics. 2016 Nov 14;17(1):913. doi: 10.1186/s12864-016-3273-2.
8
SpyB, a Small Heme-Binding Protein, Affects the Composition of the Cell Wall in .SpyB，一种小的血红素结合蛋白，影响……中细胞壁的组成。 （原文中“. ”处信息缺失）
Front Cell Infect Microbiol. 2016 Oct 13;6:126. doi: 10.3389/fcimb.2016.00126. eCollection 2016.
9
Interrupting Biosynthesis of O Antigen or the Lipopolysaccharide Core Produces Morphological Defects in Escherichia coli by Sequestering Undecaprenyl Phosphate.通过螯合十一碳烯基磷酸来中断O抗原或脂多糖核心的生物合成会在大肠杆菌中产生形态缺陷。
J Bacteriol. 2016 Oct 21;198(22):3070-3079. doi: 10.1128/JB.00550-16. Print 2016 Nov 15.
10
Dead-end intermediates in the enterobacterial common antigen pathway induce morphological defects in Escherichia coli by competing for undecaprenyl phosphate.肠杆菌共同抗原途径中的终末中间体通过竞争十一异戊二烯磷酸酯，在大肠杆菌中诱导形态缺陷。
Mol Microbiol. 2016 Apr;100(1):1-14. doi: 10.1111/mmi.13284. Epub 2015 Dec 22.
本文引用的文献
1
Development and characterization of a xylose-dependent system for expression of cloned genes in Bacillus subtilis: conditional complementation of a teichoic acid mutant.枯草芽孢杆菌中用于克隆基因表达的木糖依赖性系统的开发与特性研究：磷壁酸突变体的条件互补
Appl Environ Microbiol. 2001 Jan;67(1):403-10. doi: 10.1128/AEM.67.1.403-410.2001.
2
Incorporation of [2-3H]glycerol into cell surface components of Bacillus subtilis 168 and thermosensitive mutants affected in wall teichoic acid synthesis: effect of tunicamycin.[2-³H]甘油掺入枯草芽孢杆菌168及其在壁磷壁酸合成中受影响的温度敏感突变体的细胞表面成分：衣霉素的作用。
Microbiology (Reading). 2000 Apr;146 ( Pt 4):797-805. doi: 10.1099/00221287-146-4-797.
3
Molecular analysis of the tagF gene, encoding CDP-Glycerol:Poly(glycerophosphate) glycerophosphotransferase of Staphylococcus epidermidis ATCC 14990.编码表皮葡萄球菌ATCC 14990的CDP-甘油：聚（甘油磷酸）甘油磷酸转移酶的tagF基因的分子分析
J Bacteriol. 2000 Feb;182(4):1046-52. doi: 10.1128/JB.182.4.1046-1052.2000.
4
Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization.在野生型大肠杆菌基因组中产生精确缺失和插入的方法：应用于开放阅读框表征
J Bacteriol. 1997 Oct;179(20):6228-37. doi: 10.1128/jb.179.20.6228-6237.1997.
5
Expression, purification, and characterization of CTP:glycerol-3-phosphate cytidylyltransferase from Bacillus subtilis.枯草芽孢杆菌CTP：甘油-3-磷酸胞苷酰转移酶的表达、纯化及特性分析
J Biol Chem. 1993 Aug 5;268(22):16648-54.
6
Changes in wall teichoic acid during the rod-sphere transition of Bacillus subtilis 168.枯草芽孢杆菌168从杆状向球状转变过程中壁磷壁酸的变化
J Bacteriol. 1994 Dec;176(23):7252-9. doi: 10.1128/jb.176.23.7252-7259.1994.
7
The tagGH operon of Bacillus subtilis 168 encodes a two-component ABC transporter involved in the metabolism of two wall teichoic acids.枯草芽孢杆菌168的tagGH操纵子编码一种双组分ABC转运蛋白，参与两种壁磷壁酸的代谢。
Mol Microbiol. 1995 Apr;16(2):345-55. doi: 10.1111/j.1365-2958.1995.tb02306.x.
8
The isolation and characterization of mutants of Bacillus subtilis and Bacillus licheniformis with disturbed morphology and cell division.枯草芽孢杆菌和地衣芽孢杆菌形态与细胞分裂紊乱突变体的分离与鉴定
J Gen Microbiol. 1970 May;61(2):155-71. doi: 10.1099/00221287-61-2-155.
9
Influence of culture pH on the content and composition of teichoic acids in the walls of Bacillus subtilis.培养pH对枯草芽孢杆菌细胞壁中磷壁酸含量及组成的影响
J Gen Microbiol. 1972 Nov;73(2):395-402. doi: 10.1099/00221287-73-2-395.
10
Regulation of the bacterial cell wall: analysis of a mutant of Bacillus subtilis defective in biosynthesis of teichoic acid.细菌细胞壁的调控：枯草芽孢杆菌磷壁酸生物合成缺陷突变体的分析
J Bacteriol. 1972 Apr;110(1):281-90. doi: 10.1128/jb.110.1.281-290.1972.
Zotero 插件