Zakowich P, Morse E E
Ann Clin Lab Sci. 1975 Jan-Feb;5(1):27-30.
Platelets were obtained by differential centrifugation from Group A donors and were incubated with Na2-51Cr04. After washing to remove excess chromium, the platelets were exposed for one hour to Anti A antibody which showed agglutinating activity only. Chromium release was no greater than that of controls. Hemolytic Anti A in the presence of rabbit complement released 15 percent more chromium than controls. Anti P1A1, a platelet specific antibody, when incubated with platelets in the presence of rabbit complement released 45 percent more chromium than controls. It appears that Anti A antibody is not as destructive to platelets as a platelet specific antibody is, even when the Anti A antibody shows hemolytic activity with red cells. These observations are in agreement with previous in vivo studies of ABO incompatible platelets.
通过差速离心从A组供体获取血小板，并将其与Na2-51Cr04一起孵育。洗涤以去除多余的铬后，将血小板暴露于仅具有凝集活性的抗A抗体中一小时。铬释放量不高于对照组。在兔补体存在下的溶血型抗A释放的铬比对照组多15%。抗P1A1，一种血小板特异性抗体，在兔补体存在下与血小板一起孵育时，释放的铬比对照组多45%。看来抗A抗体对血小板的破坏作用不如血小板特异性抗体，即使抗A抗体对红细胞具有溶血活性。这些观察结果与先前关于ABO血型不相容血小板的体内研究一致。
